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Study of HER-2 Gene Amplification by Chromogenic in-situ hybridization and HER-2 Protein Amplification by IHC in Breast Cancer using Manual Tissue Microarray Sections

Suresh R., Assistant Professor, Department of Pathology, Meenakshi Medical College Hospital and Research Institute, Enathur, Kanchipuram, Tamil Nadu 631552, India. , Nagalakshmi J.a , Suresh R.b , S. Archanac , Eswari V.d

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Indian Journal of Pathology: Research and Practice 7(2):p 189-197, February 2018. | DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.7218.10

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Abstract

Aim: Breast cancer is the most common cancer in women affecting one in eight in their lifetime [1]. HER-2 gene is amplified in approximately 20-40% of breast cancers [2], which correlates with aggressive disease and amenable to treatment with Trastuzumab. The aim of this study is to detect amplification of HER-2 gene by chromogenic insitu hybridization (CISH) and HER-2 protein by immunohistochemistry in Manual Tissue microarray sections of breast cancer. Materials and Methods: 30 confirmed breast cancer cases were included in the study in which adequate tissue material were available. The breast tissue fixed in 10% buffered formalin were routinely processed and HE stained slide obtained for confirmation of tumour tissue in the paraffin block. A total of 2 Tissue microarray blocks were made each having 15 cores each from different patients using the method described by Singh et al . Sections were taken from each microarray block for HE staining, CISH and IHC study. Results: 20% showed HER-2 protein positivity. 23% were found to be positive for HER-2 gene. Cohens kappa calculated to find the degree of agreement between the HER-2 protein expression detected by IHC and HER-2 gene expression detected by CISH in Tissue microarray sections is 0.510, which indicates moderate agreement. Conclusion: We conclude HER-2 expression in carcinoma breast could be studied by IHC and CISH with ease on Tissue microarray sections, a cost effective method which can be used for both research and diagnostic purposes in resource limited laboratories.

Keywords: Breast Cancer; HER-2/neu; Chromogenic Insitu Hybridization; Immunohistochemistry; Manual Tissue Microarray.

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DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.7218.10

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