Juhi Bali PG Student, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India
Dharmesh Vasavada Professor, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India
Rashmi G.S. Phulari Professor and Head, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India
Mehul Ojha Reader, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India
Yashraj Rathore Reader, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India
Address for correspondence: Juhi Bali, PG Student, Department of Oral Pathology and Microbiology, Manubhai Patel Dental College and Hospital and ORI, Vadodara, Gujarat, India E-mail: balijuhi1234@gmail.com
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Bali J, Vasavada D, Phulari RGS, et al. Observation of Dental Structures Under the Influence of Acids: An in-Vitro Forensic Study. Indian J Forensic Med Pathol. 2025;18(2):101-109.
Timeline
Received : April 09, 2025
Accepted : June 06, 2025
Published : June 20, 2025
Abstract
Context: Forensic odontology plays a vital role in human identification, particularly when conventional methods are hindered by decomposition or trauma. Understanding the effects of various environmental factors on teeth is crucial for effective forensic analysis.
Aims: The study aim to evaluate the effects of three different acids - 37% Hydrochloric Acid (HCl), 65% Nitric Acid (HNO3), and 98% Formic Acid (HCOOH) - on extracted permanent mandibular first molars, focusing on noncarious, carious, and Class I composite filled teeth.
Methods and Material: A total of 45 extracted permanent mandibular first molars were subjected to acid solutions each of 30 ml for different durations ranging from 15 minutes to 24 hours. The teeth were categorized into non-carious, carious, and Class I composite-filled groups. Morphological, microscopic, and radiographic evaluations were conducted at various time intervals to assess the effects of acid exposure on the dental tissues and restorations.
Results: The study found that acid exposure led to significant changes in the morphology, microscopic structure, and radiographic appearance of both dental tissues and restorations. Variations in the rate of degradation were observed depending on the type of acid and the condition of the teeth.
Conclusions: The results offer valuable insights into the effects of acid exposure on teeth, particularly in forensic odontology. Understanding these effects is critical for evaluating the durability of dental structures and restorations in forensic identification processes, where teeth are frequently used as a primary means of identification.
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Funding
This research received no funding.
Author Contributions
All authors contributed significantly to the work and approve its publication.
Ethics Declaration
This article does not involve any human or animal subjects, and therefore does not require ethics approval.
Acknowledgements
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Conflicts of Interest
The authors report no conflicts of interest in this work.
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Cite this article
Bali J, Vasavada D, Phulari RGS, et al. Observation of Dental Structures Under the Influence of Acids: An in-Vitro Forensic Study. Indian J Forensic Med Pathol. 2025;18(2):101-109.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.