Full Text (PDF)
Original Article

Observation of Dental Structures Under the Influence of Acids: An In-Vitro Forensic Study

Juhi Bali, Dharmesh Vasavada, Rashmi G.S. Phulari, Mehul Ojha, Yashraj Rathore

Author Information

Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.


Indian Journal of Forensic Medicine and Pathology 18(2):p 101-109, Jan-June 2025. | DOI: https://doi.org/10.21088/ijfmp.0974.3383.18225.3

How Cite This Article:

Bali J, Vasavada D, Phulari RGS, et al. Observation of Dental Structures Under the Influence of Acids: An in-Vitro Forensic Study. Indian J Forensic Med Pathol. 2025;18(2):101-109.

Timeline

Received : April 09, 2025         Accepted : June 06, 2025          Published : June 20, 2025

Abstract

Context: Forensic odontology plays a vital role in human identification, particularly when conventional methods are hindered by decomposition or trauma. Understanding the effects of various environmental factors on teeth is crucial for effective forensic analysis. Aims: The study aim to evaluate the effects of three different acids - 37% Hydrochloric Acid (HCl), 65% Nitric Acid (HNO3), and 98% Formic Acid (HCOOH) - on extracted permanent mandibular first molars, focusing on noncarious, carious, and Class I composite filled teeth. Methods and Material: A total of 45 extracted permanent mandibular first molars were subjected to acid solutions each of 30 ml for different durations ranging from 15 minutes to 24 hours. The teeth were categorized into non-carious, carious, and Class I composite-filled groups. Morphological, microscopic, and radiographic evaluations were conducted at various time intervals to assess the effects of acid exposure on the dental tissues and restorations. Results: The study found that acid exposure led to significant changes in the morphology, microscopic structure, and radiographic appearance of both dental tissues and restorations. Variations in the rate of degradation were observed depending on the type of acid and the condition of the teeth. Conclusions: The results offer valuable insights into the effects of acid exposure on teeth, particularly in forensic odontology. Understanding these effects is critical for evaluating the durability of dental structures and restorations in forensic identification processes, where teeth are frequently used as a primary means of identification.


References

  • 1.   Johnson A., Gandhi B., Joseph S.E. A morphological study of tongue and its role in forensics odontology. J Forensic Sci & Criminal Inves. 2018; 7(5): 1-5.
  • 2.   Gupta J., Gupta K.K., Kabiraj A., Samadi F.M., Karki R.S., Yadav S. Morphological analysis of extracted human teeth in three different acids as an aid in forensic identification. World j Pharm Res. 2015Aug 4; 4(10): 1982-92.
  • 3.   Saxena S., Sharma P., Gupta N. Experimental studies of forensic odontology to aid in the identification process. Journal of forensic dental sciences. 2010 Jul; 2(2): 69.
  • 4.   Whittaker D.K. Research in forensic odontology. Annals of the Royal College of Surgeons of England. 1982 May; 64(3): 175.
  • 5.   Eckert W.G. Chapter 1. In: Introduction to the forensic sciences, ed by Eckert WG, CRC press, 1997, pp. 1-10.
  • 6.   Dinakaran J., Dineshkumar T., Nandhini G, Priyadharshini N, Rajkumar K. Gender determination using dentition. SRM Journal of Research in Dental Sciences. 2015 Jan 1; 6(1): 29-34.
  • 7.   Gupta K.K., Johnson A. Morphologic and radiographic effects of acids on the teeth: An invitro forensic study. Indian Journal of Forensic Medicine & Toxicology. 2020 Jan 16; 14(1): 28-33.
  • 8.   Seethapathy T., Shylaja S., Sekhar M.S., Manthapuri S., Ramanand OV, Reddy SK, Paul JB, Funato A, Kubo K, Maeda H, Alam MK. Effect of acids on teeth and restorative materials: An aid in forensic odontology. Journal of Hard Tissue Biology. 2019; 28(1): 21-30.
  • 9.   Kadashett V., Shivakumar K.M., Baad R., Vibhute N., Belgaumi U., Bommanavar S., Kamate W. Effect of Concentrated Acids on Teeth: A Forensic Approach; An: In-vitro: Study. Journal of Datta Meghe Institute of Medical Sciences University. 2021 Apr 1; 16(2): 283-9.
  • 10.   Raj M., Boaz K., Srikant N. Are teeth evidence in acid environment? Journal of Forensic Dental Sciences. 2013 Jan 1; 5(1): 7-10.
  • 11.   Maladkar S.R., Yadav P., Muniraja A.N., Uchil G.S., George L.V., Augustine D., Rao R.S., Patil S., Sowmya S.V., Haragannavar V.C. Erosive Effect of Acidic beverages and Dietary preservatives on extracted human Teeth—An in Vitro Analysis. European Journal of Dentistry. 2022 Oct; 16(04): 919-29.
  • 12.   Cope D.J., Dupras T.L. The effects of household corrosive chemicals on human dentition. Journal of forensic sciences. 2009 Nov; 54(6): 1238-46.
  • 13.   Mazza A., Merlati G., Savio C., Fassina G., Menghini P., Danesino P. Observations on dental structures when placed in contact with acids: Experimental studies toaididentification processes. Journal of Forensic and Sciences. 2005 Mar 1; 50(2): JFS2004292-5.

Data Sharing Statement

There are no additional data available.

Funding

This research received no funding.

Author Contributions

All authors contributed significantly to the work and approve its publication.

Ethics Declaration

This article does not involve any human or animal subjects, and therefore does not require ethics approval.

Acknowledgements

Information not provide.

Conflicts of Interest

The authors report no conflicts of interest in this work.


About this article


Cite this article

Bali J, Vasavada D, Phulari RGS, et al. Observation of Dental Structures Under the Influence of Acids: An in-Vitro Forensic Study. Indian J Forensic Med Pathol. 2025;18(2):101-109.


Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.


Received Accepted Published
April 09, 2025 June 06, 2025 June 20, 2025

DOI: https://doi.org/10.21088/ijfmp.0974.3383.18225.3

Keywords

Forensic OdontologyHuman IdentificationAcid ExposureMorphological ChangesMicroscopic Evaluation

Article Level Metrics

Last Updated

Wednesday 17 June 2026, 23:17:12 (IST)


7105

Accesses

26
2030
00

Citations


NA
NA
NA

Download citation


Article Keywords


Keyword Highlighting

Highlight selected keywords in the article text.


Timeline


Received April 09, 2025
Accepted June 06, 2025
Published June 20, 2025

licence


Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.


Access this article



Share