Abstract

Rajasthan’s southeastern part is known as “the land of Hada (Clan) Rajputs” i.e Hadoti. In this study, genetic structure of Hadoti population, who exhibited diverse ethanographic past, have been explored using 23 autosomal STR loci [PowerPlex® Fusion 6C (PPF) system] by the way of determining allele distribution and forensic efficiency parameters. Autosomal STR data was collected from the blood samples of 423 (194 females and 229 males) individuals of Rajasthani roots related to the Hadoti region. The STRs were amplified by using PPF 6C system (Promega Corporation) and the amplified samples were examined using Gene Mapper ID-X 1.6 software (Applied Biosystems) on a 3500xl Genetic Analyzer. Following Bonferroni correction, all the studied STR loci fulfilled the terms of the HardyWeinberg equilibrium. With the exception of Amelogenin and DYS391, DYS570, DYS576, this study found that among the 23 autosomal STR loci, SE 33 is the most informative (power of discrimination (PD) = 0.992), whereas TPOX locus (PD = 0.866)is the least. The obtained parameters of forensic interest demonstrated the compatibility of all the studied 23 STRs across Hadoti population of Rajasthan for forensic DNA identification and paternity testing.

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