Full Text (PDF)
Review Article

Comparative Study on Touch DNA Extraction Method

Priyanka Chhabra, Sudhanshu Sawhney1 null, Kajol Bhati2 null, Unnati Gupta3 null

Author Information

Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.



Indian Journal of Forensic Medicine and Pathology 14(2 (Special Issue)):p 374-378, April-June 2021. | DOI: 10.21088/ijfmp.0974.3383.14221.52

How Cite This Article:

Sawhney S. Comparative study on touch DNA extraction method. Indian J Forensic Med Pathol. 2021;14(3 Special):374-8.

Timeline

Received : April 02, 2021         Accepted : April 20, 2021          Published : June 30, 2021

Abstract

Touch DNA viz. trace DNA is a perfect example of Locard’s exchange principle, formulating the basis of forensic science. It basically states that any contact between two objects exchanges a trace amount of matter. Touch DNA is attained from the shed skin cells whenever a person comes in contact or touches any object. This kind of DNA can be found in every sample of fingerprint but its extraction is a cumbersome task in consideration of its trace amount. There is diversity in methods of its extraction but the main problem arises when task is to preserve fingerprint and extract DNA as well. Scientifically, the evidentiary value of fingerprints is 100% and that of DNA is 99.9%. It becomes crucial to preserve both of them to strengthen our case. In this era of vicious and cunning criminals where evidences are obscured. The extraction of touch DNA is practical but not practiced. This study aims at comparing various methods and finding best one out. The most appropriate method should be able to analyze the smallest possible amount of DNA in an economic way and of course leaving the fingerprint indifferent


References

  • 1.   Bhoelai B, Beemster F, Sijen T. Revision of the tape used in a tape-lift protocol for DNA recovery. Forensic Sci Int Genet Suppl Ser. 2013;4(1):e270-1.
  • 2.   Daly DJ, Murphy C, McDermott SD. The transfer of touch DNA from hands to glass, fabric and wood. Forensic Sci Int Genet. 2012;6(1):41-6.
  • 3.   Hess S, Haas C. Recovery of trace DNA on clothing: a comparison of mini-tape lifting and three other forensic evidence collection techniques. J Forensic Sci. 2017;62(1):187-91.
  • 4.   Kirgiz IA, Calloway C. Increased recovery of touch DNA evidence using FTA paper compared to conventional collection methods. J Forensic Leg Med. 2017;47:9-15.
  • 5.   Kopka J, Leder M, Jaureguiberry SM, Brem G, Boselli GO. New optimized DNA extraction protocol for fingerprints deposited on a special self-adhesive security seal and other latent samples used for human identification. J Forensic Sci. 2011;56(5):1235-40.
  • 6.   Pang BCM, Cheung BKK. Double swab technique for collecting touched evidence. Leg Med (Tokyo). 2007;9(4):181-4.
  • 7.   Solomon AD, Hytinen ME, McClain AM, Miller MT, Dawson Cruz T. An optimized DNA analysis workflow for the sampling, extraction, and concentration of DNA obtained from archived latent fingerprints. J Forensic Sci. 2018;63(1):47-57.
  • 8.   Tang J, Ostrander J, Wickenheiser R, Hall A. Touch DNA in forensic science: the use of laboratory-created eccrine fingerprints to quantify DNA loss. Forensic Sci Int Synerg. 2020;2:1-16.
  • 9.   van Helmond W, van O'Brien V, de Jong R, van Esch J, de Puit M. Collection of amino acids and DNA from fingerprints using hydrogels. Analyst. 2018;143(4):863-70.
  • 10.   Verdon TJ, Mitchell RJ, van Oorschot RAH. Evaluation of tapelifting as a collection method for touch DNA. Forensic Sci Int Genet. 2014;8(1):179-86.
  • 11.   Schiffner LA, Bajda EJ, Prinz M, Sebestyen J, Shaler R, Caragine TA. Optimization of a simple, automatable extraction method to recover sufficient DNA from low copy number DNA samples for generation of short tandem repeat profiles. Croat Med J. 2005;46(4):578-86.
  • 12.   Lowe A, Murray C, Richardson P, Wivell R, Gill P, Tully G, et al. Use of low copy number DNA in forensic inference. Int Congr Ser. 2003;1239:799-801.
  • 13.   de Bruin KG, Verheij SM, Veenhoven M, Sijen T. Comparison of stubbing and the double swab method for collecting offender epithelial material from a victim’s skin. Forensic Sci Int Genet. 2012;6(2):219-23.
  • 14.   Kanokwongnuwut P, Kirkbride P, Linacre A. Visualising latent DNA on swabs. Forensic Sci Int. 2018;291:115-23.
  • 15.   Plaza DT, Mealy JL, Lane JN, Parsons MN, Bathrick AS, Slack DP. Nondestructive biological evidence collection with alternative swabs and adhesive lifters. J Forensic Sci. 2016;61(2):485-8.
  • 16.   Wickenheiser RA. Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. J Forensic Sci. 2002;47(3):442-50.
  • 17.   Verdon TJ, Mitchell RJ, van Oorschot RAH. Evaluating the efficiency of DNA extraction methods from different substrates. Forensic Sci Int Genet Suppl Ser. 2011;3(1):e93-4.
  • 18.   Chow JFC, Yeung WSB, Lee VCY, Lau EYL, Ng EHY. PGT-SR using NGS and haplotype analysis with microsatellite markers to distinguish normal from balanced Robertsonian translocation carrier embryo. Reprod Biomed Online. 2019;38(Suppl 1):e21-2.
  • 19.   Phipps M, Petricevic S. The tendency of individuals to transfer DNA to handled items. Forensic Sci Int. 2007;168(2-3):162-8.
  • 20.   Thamnurak C, Bunakkharasawat W, Riengrojpitak S, Panvisavas N. DNA typing from fluorescent powder dusted latent fingerprints. Forensic Sci Int Genet Suppl Ser. 2011;3(1):e524-5.
  • 21.   Schulz MM, Reichert W. Archived or directly swabbed latent fingerprints as a DNA source for STR typing. Forensic Sci Int. 2002;127(1-2):128-30.
  • 22.   Kamphausen T, Fandel SB, Gutmann JS, Bajanowski T, Poetsch M. Everything clean? Transfer of DNA traces between textiles in the washtub. Int J Legal Med. 2015;129(4):709-14.
  • 23.   Gunnarsson J, Eriksson H, Ansell R. Success rate of a forensic tape-lift method for DNA recovery. Probl Forensic Sci. 2010;83:243-54.
  • 24.   Sweet D, Lorente M, Lorente JA, Valenzuela A, Villanueva E. An improved method to recover saliva from human skin: the double swab technique. J Forensic Sci. 1997;42(2):320-2.
  • 25.   van Oorschot RAH, Weston RK, Jones MK. Retrieval of DNA from touched objects. In: Proceedings of the 14th International Symposium on the Forensic Sciences; 1998 Oct 12-16; Adelaide, Australia.
  • 26.   Milne E, van Bockxmeer FM, Robertson L, Soutar D, Dadour I. Buccal DNA collection: comparison of buccal swabs with FTA cards. Cancer Epidemiol Biomarkers Prev. 2006;15(4):816-9.
  • 27.   Poy AL, van Oorschot RAH. Trace DNA presence, origin, and transfer within a forensic biology laboratory and its potential effect on casework. J Forensic Identif. 2006;56(4):558-76.
  • 28.   Ladd C, Adamowicz MS, Bourke MT, Scherczinger CA, Lee HC. A systematic analysis of secondary DNA transfer. J Forensic Sci. 1999;44(6):1270-2.
  • 29.   Lowe A, Murray C, Whitaker J, Tully G, Gill P. The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci Int. 2002;129(1):25-34.

About this article


Cite this article

Sawhney S. Comparative study on touch DNA extraction method. Indian J Forensic Med Pathol. 2021;14(3 Special):374-8.


Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.



Received Accepted Published
April 02, 2021 April 20, 2021 June 30, 2021

DOI: 10.21088/ijfmp.0974.3383.14221.52

Keywords

touch dnafingerprinttrace evidenceextraction

Article Level Metrics

Last Updated

Wednesday 08 July 2026, 04:21:23 (IST)


7722

Accesses

1
2137
00

Citations


NA
NA
NA

Download citation


Article Keywords


Keyword Highlighting

Highlight selected keywords in the article text.


Timeline


Received April 02, 2021
Accepted April 20, 2021
Published June 30, 2021

licence


Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.



Access this article



Share