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Optimizing Phenol-Chloroform Extraction Method for Human Papilloma Virus (HPV) Genomic DNA Isolation from Fresh Cervical Tissue

B.R. Sontakke, Assistant Professor, Department of Anatomy, Mahatma Gandhi Institute of Medical sciences, Sevagram, Wardha, Maharashtra-442102, India. , B.R. Sontakke* , P. Ambulkar** , S. Talhar* , J.E. Waghmare*** , Poonam Varma Shivkumar**** , V.B. Shivkumar***** , A.K. Pal******

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Indian Journal of Anatomy 6(2):p 165-167, April- June 2017. | DOI: http://dx.doi.org/10.21088/ija.2320.0022.6217.12

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Abstract

Our aim was to optimize a cost effective, efficient protocol for genomic DNA extraction of HPV from exfoliated cells of cervix. The final goal was to get a high quality and quantity DNA yield. The DNA obtained was confirmed by horizontal gel assembly and visualizing the same by Gel Doc System. The optimized protocol for genomic DNA extraction of HPV is good for laboratory with low funds or budget.


Keywords: DNA; Extraction; HPV; Cervical Cells.  

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DOI: http://dx.doi.org/10.21088/ija.2320.0022.6217.12

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