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Novel Technique for Rapid Screening of Malarial Parasite by Toluidine Blue Staining

Vissa Shanthi, Professor, Department of pathology, Narayana Medical college and Hospital, Nellore, Andhra Pradesh 524003, India , Bheemaraju Venkata Vydehi1 , Vissa Shanthi2 , Nandam Mohan Rao3 , Byna Syam Sundara Rao4 , Bhavana Grandhi5 , Saumya Bandla6

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Indian Journal of Pathology: Research and Practice 7(10):p 1053-1056, October 2018. | DOI: DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.71018.1

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Abstract

Introduction: Malaria is a tropical disease causing millions of death worldwide. Early detection and management prevents complications and mortality. Definitive diagnosis depends on demonstration of malaria in blood smears. Aims & objectives: In this study we intend to evaluate Toluidine blue utility and efficacy in screening & species identification of malaria parasite in peripheral smear. Materials and methods: In 134 patients with clinical suspicion of malaria, peripheral smears were prepared and were processed with both conventional Leishman method and Toluidine blue method simultaneously. Results: Out of 134 smears, 51 were positive for malaria with male preponderance and age incidence being more between 41-50 yrs. Predominant species was Plasmodium vivax (76.47%). Leishman stain showed decreased sensitivity (84.3%) and negative predictive value (91.2%) when compared to Toluidine blue stain. Conclusion: Toluidine blue staining method helps to identify parasites even at low power objective allowing screening more areas in shorter time. It is specifically useful in low parasite index cases and in species identification 
 
Keywords: Malaria Parasite; Leishman Stain; Toluidine Blue; Peripheral Smear.

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DOI: DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.71018.1

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