Home > Journals > Journal of Microbiology and Related Research

Journal of Microbiology and Related Research

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Nitric Oxide Production in Probiotic lactobacillus Plantarum : Revision of the Origin

Dina R. Yarullina , Dina R. Yarullina , Knut Beuerlein , Nikolay I. Silkin , Olga N. Ilinskaya

Author Information

Licence:



Journal of Microbiology and Related Research 1(1):p 5-14, January - June 2015. | DOI:

How Cite This Article:

Timeline

Received : N/A         Accepted : N/A          Published : N/A

Abstract

 Nitric oxide (NO) is formed in gastrointestinal tract by both intestinal mucosa and commensal microbiota. In this work, NO production by probiotic bacteria Lactobacillus plantarum 8PA3 was demonstrated by electron paramagnetic resonance (EPR)and NOspecificfluorescent dyes DAF-FM DA (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) and DAA (1,2-diaminoanthraquinone sulfate). After we ruled out the generation of NO via denitrification, we showed that NO production by L. plantarumdepends on L-arginine what is typical for NO-synthases (NOS), and thus proposed NOS-activity in L. plantarum. However,we were unable to inhibit the NO production with three specific NOS inhibitorsL-NAME, L-NIL, and nNOS Inhibitor I.We also demonstrated an increased level of NO production in lactobacilli with damaged cellular membranes suggesting stress-dependent NO production by L. plantarum.

Keywords: Lactobacillus plantarum,denitrification, nitric oxide synthase (NOS), electron paramagnetic resonance (EPR), fluorescent staining.

References

No records found.


About this article

Cite this article

Licence:



Received Accepted Published
N/A N/A N/A
DOI:

Keywords


Article Level Metrics

Last Updated

Monday 21 September 2026, 14:30:19 (IST)

1469

Accesses

8
188
00

Citations


NA
NA
NA

Download citation


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article

Reprint Order Form
Buy this Article
Institutional Subscriptions

Share