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Evaluation of the Efficacy of Bacterial Collagenase in Wound Healing

Vijay Prabha, Professor, Department of Microbiology, Panjab University, Chandigarh 160014, India. , Sofica Rana1 , Thomson Soni2 , Aditi Chauhan3 , Vijay Prabha4

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Journal of Microbiology and Related Research 8(2):p 45-49, July-December 2022. | DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.8222.2

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Sofia Rana, Thomson Soni, Aditi Chauhan, et. al./Evaluation of the Efficacy of Bacterial Collagenase in Wound Healing/J
Microbiol Relat Res. 2022;8(2): 45–49.

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Received : N/A         Accepted : N/A          Published : N/A

Abstract

Introduction: The peptides resulting from collagen degradation by collagenases have a wide range of applications including wound healing. However, most of the wound healing studies involve collagenase obtained from anaerobic and pathogenic microorganisms thus increasing the production cost of the same.

Methodology: Earlier in our laboratory, collagenase was isolated and purified from aerobic, non-pathogenic Bacillus altitudinis and it has shown excellent activity in vitro. Thus, the present study was carried out to evaluate the wound-healing potential of collagenase isolated and purified from Bacillus altitudinis in a murine model. For this, male mice were divided into 2 groups viz. test group (n=6) and control group (n=6), and a burn wound was created on the dorsal side of each mouse with the help of a heated brass bar after exposing the skin. In the case of the test group purified collagenase (10µg/100 µl) was applied on the burn wound while phosphate-buffered saline (100 µl) was applied in the case of a control group for 5 consecutive days. On day 6th the mice (n =3) were sacrificed from each group and wound biopsies were collected for histopathological examinations

Results: Histopathological examinations showed the absence of epithelium in the control group whereas in the test group initial signs of regeneration of the epidermis were observed. The wound recovery when examined visually and by calculating the wound area by using a caliper the result showed that the wound was fully recovered. Conclusion: Purified collagenase can be used as a therapeutic measure in burn wound healing.

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Cite this article

Sofia Rana, Thomson Soni, Aditi Chauhan, et. al./Evaluation of the Efficacy of Bacterial Collagenase in Wound Healing/J
Microbiol Relat Res. 2022;8(2): 45–49.

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DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.8222.2

Keywords

Collegenase; Bacillus altitudinis; Burn wound; Mouse model; Histopathological examination.

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