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Diagnosing Mycobacterium tuberculosis by smear, culture and Real time PCR technique in pleural fluid samples

Heena Rajani , Heena Rajani

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New Indian Journal of Surgery 2(4):p 281-281, October-December 2011. | DOI:

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Abstract

 Tuberculous pleural effusion is one of the most common forms of extra pulmonary tuberculosis. For its definitive diagnosis, getting pleural fluid as smear positive followed by culture positive for Mycobacterium tuberculosis is traditionally considered the gold standard. However the utility of these conventional tests is tedious and has a low sensitivity in pleural fluid samples including the delay in obtaining the results. As reported by various studies, the sensitivity (31.3–81%) and specificity (96.6–100%) of PCR are variable. This study was undertaken to evaluate the utility of a molecular tool in the reconfirming a clinical diagnosis of pleural tuberculosis.

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