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Colonization of Aspergillus Species in COPD Patients and Their Antifungal Suceptibility

Priyanka Choudhary* , Priyanka Choudhary* , Pooja Singh Gangania*

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Journal of Microbiology and Related Research 3(1):p 37-41, January - March 2017. | DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.6

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Abstract

 Objectives: To isolate Aspergillus species from lung infection in COPD patients and to determine Antifungal Susceptibilty against Amphotercin B by E-Test Strip method. Materials and Method: Sputum sample collected from total 54 patients visiting TB and Chest department of Santosh Hospital. All are above 18 year of Age and suffering from COPD. Aspergillus species were isolated by culturing samples on SDA which were confirmed by conventional method. Antifungal Susceptibilties were determined by E-Test strip method on Muller Hinton Agar with Methylene Blue Dye . E-Test minimum inhibitory concentrations (MIC) of Amphotericin B determined. Result: 33% of Aspergillus species were isolated from sputum samples of COPD patients. Conclusion: The analysis of the present study concludes that Aspergillus is one of the major cause of colonization in COPD patients,especially males within age group( 41-60) years, chronic smoking increases the rate of Aspergillus colonization and Amphotericin B gives a poor result in treatment.

Keywords: Chronic Obstructive Pulmonary Disease; Aspergillus Fumigatus ; Aspergillus Flavus ; Aspergillus Niger; Aspergillus Terreus; Lactophenol Cotton Blue; Sabourad’s Dextrose Agar; Potato Dextrose Agar; Minimum Inhibitory Concentration; Elipsometer Test.

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DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.6

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