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A Study on Antibacterial Activity of Crude Ethanolic Fruit Peel Extracts against Bacterial Isolates by well Diffusion Method and Analysis of Punica Granatum by Thin Layer Chromatography

Shajitha Fathima, , R. Ushasri1 , Shajitha Fathima2

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Journal of Microbiology and Related Research 11(1):p 21-27, Jan-June 2025. | DOI: 10.21088/jmrr.2395.6623.11125.3

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R. Ushasri, Shajitha Fathima. A Study on Antibacterial Activity of Crude Ethanolic Fruit Peel Extracts against Bacterial Isolates by well Diffusion Method and Analysis of Punica Granatum by Thin Layer Chromatography. J Microbiol Relat Res. 2025; 11(1): 21–27.
 

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Abstract

Milk has strong sensory qualities and has all the nutrients needed for the growth of all microorganisms. Additionally, milk is thought to prevent or lower the chances of certain illnesses linked to nutritional deficiencies. raw, unpasteurized milk contains a wide variety of microorganisms, including Salmonella, Campylobacter jejuni, Escherichia coli, and Aeromonas, all of which are linked to health problems.  Citrus fruit contains sugar, organic acids, lipids, polysaccharides, vitamins, minerals, flavonoids, carotenoids, and volatile compounds. The aim of our study was to To determine the antibacterial activity of crude ethanolic fruit peel extracts against bacterial isolates and to analyse components in the fruit peel extract by thin layer chromatography. The Orange,Sweet lime and pomegranate peels were collected from koyambedu market Chennai..The crude ethanolic extracts were prepared and antibacterial activity was performed by well diffusion technique. The methanol ethanolic and aqueous extracts of pomegranate exhibited antibacterial activity against Listeria, S.aureus and salmonella spp with zone of inhibition ranging from 19mm at concentration of 15 mg/ml and 25 mg/ml. The presence study showed lowest antibacterial activity against salmonella typhi, Bacillus species with zones of inhibition as 15mm and 14mm at a concentration of 500μl/ml and 250μl/ ml by well diffusion method. The current study revealed RF values of two fractions a and b as 0.11 and 0.93.

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R. Ushasri, Shajitha Fathima. A Study on Antibacterial Activity of Crude Ethanolic Fruit Peel Extracts against Bacterial Isolates by well Diffusion Method and Analysis of Punica Granatum by Thin Layer Chromatography. J Microbiol Relat Res. 2025; 11(1): 21–27.
 

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DOI: 10.21088/jmrr.2395.6623.11125.3

Keywords

• Fruit peel extract • Well diffusion technique • Antibacterial activity • Carotenoids

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