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RFP Journal of Biochemistry and Biophysics

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Study of Oxidative Stress in Smokers by Estimation of Serum Malondialdehyde Uric Acid and Bilirubin

Raviraj Rajan Naik, IB 66, OM Bungalow, Behind Nath Seeds, Paithan Road, Aurangabad – 431005 Maharashtra, India. , Raviraj Naik1 , Savita Deshmukh1 , Sarita Dakhure2

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RFP Journal of Biochemistry and Biophysics 2(1):p 17-21, January - June 2017. | DOI:

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Abstract

Background: Study of Malondialdehyde Uric Acid and Bilirubin smokers provides opportunity to explain the correlation between cigarette smoking activity and oxidative stress. Cigarette smoking is the second cause of death in the world. Cause oxidative stress, which overwhelm natural radical blocking or scavenging mechanisms. Cigarette smoke may promote atherogenesis by producing oxygenderived free radicals that damage lipids. Cigarette smoking is associated with impaired endotheliumdependent vasodilatation and cardiovascular disease (CVD). As the current report concerns solely to the study oxidative stress in smokers, the results of this study can be correlated with other biochemical, physiological and clinical aspects. Aim: The Aim of our study was to determine oxidative stress in smokers by estimation of serum Malondialdehyde , Uric Acid and Bilirubin in smokers as compared to nonsmokers. Method: A Total No. of 100 subjects were selected, out of which 50 were healthy individual and 50 were smokers less than 50 years of age. Uric acid and Bilirubin were analyzed using kits on automated biochemistry analyzer while MDA was estimated spectrophotometrically using Thiobarbituric acid. Result: The levels of Malondialdehyde were significantly higher while the levels of Uric Acid and Bilirubin were significantly lower in smokers as compared to their levels in non smokers.

Keywords: Malondialdehyde Uric Acid and Bilirubin; Smokers; NonSmokers. 

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