Home > Journals > Journal of Pharmaceutical and Medicinal Chemistry

Journal of Pharmaceutical and Medicinal Chemistry

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Novel Approach of Stability Indicating Method Development for Determination of Metformin and Empagliflozin by High Performance Liquid Chromatography

A Sai Datri, Assistant Professor, Department of Pharmaceutical Analysis, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh 521356, India.ajay.pharma3006@gmail.com. , A Sai Datri1 , A Lakshmana Rao2 , Ch Purna Durganjali3

Author Information

Licence:



Journal of Pharmaceutical and Medicinal Chemistry 8(2):p 53-61, July-December 2022. | DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.8222.1

How Cite This Article:

A Sai Datri, A Lakshmana Rao, Ch Purna Durganjali/Novel Approach of Stability Indicating Method Development for Determination of Metformin and Empagliflozin by High Performance Liquid Chromatography/ J Pharmaceut Med Chem. 2022;8(2):53-61

Timeline

Received : N/A         Accepted : N/A          Published : N/A

Abstract

A simple, accurate, precise RP-HPLC method was developed for the simultaneous estimation of the Metformin and Empagliflozin in the tablet dosage form. Materials and Methods: Chromatogram was run through Std Agilent 18 (150 x 4.6mm, 5μm). Mobile phase taken as 0.1% OPA Buffer: Acetonitrile in 60:40% v/v ratio, and pumped through the column at a flow rate of 1mL/min. The buffer used in this method was 0.1% OPA buffer. The temperature was maintained at 25°C. Results and Discussion: Optimized wave length selected was 245 nm. The retention times of Metformin and Empagliflozin were found to be 2.193 min and 2.668 min respectively. %RSD of the Metformin and Empagliflozin was found to be 1.4 and 0.8 respectively. % Recovery was obtained as 99.66% and 100.24% for Metformin and Empagliflozin respectively. LOD, LOQ values obtained from regression equations of Metformin and Empagliflozin were 0.02, 1.48, and 0.05, 4.93 respectively. Regression equation of Metformin is y = 20952x + 9914.5 and y = 41842x + 571.79 of Empagliflozin. Retention times were decreased and that run time was decreased, The Reverse Phase HPLC isocratic method for Metformin and Empagliflozin is developed and validated as per ICH guidelines. Conclusion: The test method is found to be sensitive, accurate, precise, linear, convenient, and economical that can be adopted in regular quality control tests in Industries..

References

No records found.


About this article

Cite this article

A Sai Datri, A Lakshmana Rao, Ch Purna Durganjali/Novel Approach of Stability Indicating Method Development for Determination of Metformin and Empagliflozin by High Performance Liquid Chromatography/ J Pharmaceut Med Chem. 2022;8(2):53-61

Licence:



Received Accepted Published
N/A N/A N/A
DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.8222.1

Keywords

Metformin; Empagliflozin; RP-HPLC; Validation.

Article Level Metrics

Last Updated

Monday 22 June 2026, 07:50:09 (IST)

437

Accesses

3
28
00

Citations


NA
NA
NA

Download citation


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article

Reprint Order Form
Buy this Article
Institutional Subscriptions

Share