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Molecular Characterization of Chikungunya Virus in Serum- Relevance for Disease Management

Sharma Narotam, Scientist, Central Molecular Research Laboratory, Shri Guru Ram Rai Institute of Medical and Health Sciences, SGRR University, Dehradun, Uttarakhand 248001, India. , Sharma Narotam 1 , Gupta Komal 2 , KumarVijay 3 , Ghanshyam 4 , Rawat Anita 5 , Saha Shiny 6

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Indian Journal of Genetics and Molecular Research 7(1):p 13-15, January- June 2018. | DOI: http://dx.doi.org/10.21088/ijgmr.2319.4782.7118.2

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Abstract

Background: Chikungunya Virusis a single stranded RNA virus of the genus alphavirus.
The transmission of this virus occurs by the bite of infected mosquitoes. It is characterized by an  brupt onset of fever frequently accompanied by joint pain. 

Aims: Molecular Characterization of  Chikungunya Virus in Serum- Relevance for Disease Management. 

Methods and Materials: 30 cases were considered having symptom of fever suspected to have chikungunya. RNA was isolated from magnetic beads methods which was further studied for Real Time-PCR (RT-PCR).

Results: Among the 30 cases suspected for chikungunya fever, only 18 were positive for the same. Hence, the prevalence was calculated to be 60%. About 39% of the positive cases were in the age range of 21-40 years, which is the active age group of the total positive cases, 66.66% were males and 33.33% were females. The virus can be even transmitted together as a co-infection. Patients infected with chikunguniya virus are more likely to experience symptoms of high fever, severe polyarthralgia and rash with lymphopenia.


 

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DOI: http://dx.doi.org/10.21088/ijgmr.2319.4782.7118.2

Keywords

ChikunguniyaReal Time Polymerase Chain Reaction; Aedes Aegypti; Aedes Albopictus

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