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Indian Journal of Pathology: Research and Practice

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Evaluation of Role of Touch Cytology in Diagnosis of Helicobacter Pylori in Non-Ulcer Dyspepsia Cases

Udita Singhal, Assistant Professor, Department of Pathology, ESIC Dental College & Hospital, Rohini, Sector - 15, Delhi 110089, India. , Udita Singhala , C.L. Pandeyb , Sangeeta Lambac , Annu Nandad

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Indian Journal of Pathology: Research and Practice 7(3):p 384-390, March 2018. | DOI: DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.7318.18

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Abstract

H. pylori has emerged as an undisputed cause of chronic active gastritis, however the relationship between H. pylori and non- ulcer dyspepsia is still a matter of controversy. Various tests are available for diagnosis of H. pylori. Research in several directions has brought forward no general consensus about the most suitable method to diagnose H. pylori. Hence this study was done to evaluate the role of touch cytology diagnosis of H. pylori infection in patients of non -ulcer dyspepsia and to compare it with histology and urease test. Material And Methods: Touch smears were made from antralbiopsies obtained from patients (125) undergoing upper GI endoscopy. Rapid urease test was performed. Touch smears were stained with Giemsa stain and histological sections with Haemtoxylin and Eosinstain. The gastric biopsies were classified as per Sydney system. Observations and Results: In this study touch cytology was positive for H pylori in 53 out of 83 cases (62.8%). Organisms were better appreciated on smears than on the biopsies, smears took lesser time to screen and report was available to the patient and the clinician the same day. Urease test was positive in 57.8% cases making it a reliable and rapid test for diagnosis. The percentage positivity ofit is comparable to touch cytology results (62.8%) in our study. Thus touch smear cytology appears to complement histopathology. Conclusions: Touch cytology is a quick and simple sensitive test for diagnosis of H. pylori infection in patients of non- ulcer dyspepsia. It complements histology and urease test. 

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DOI: DOI: http://dx.doi.org/10.21088/ijprp.2278.148X.7318.18

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