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Detection of CTXB Gene by PCR

K S N Ravi Teja , K S N Ravi Teja

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New Indian Journal of Surgery 2(4):p 293-293, October-December 2011. | DOI:

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Abstract

 The culture based techniques in isolation of Vibrio cholera have disadvantage like requirement of upto 72hrs for completion ,failure to detect viable but nonculturable (VNBC) cells and some difficulties arising where Vibrio cholerae are outcompeted by other bacteria during enrichment and subculturing steps. Thus trials for detection of ctxb gene using molecular technique like Polymerase Chain Reaction (PCR) were done by us which was rapid, sensitive, highly selective and did not require extensive hands on time. As a preliminary step, we wanted to check wether clinical isolates produced from culture of stool amplified the toxin gene in PCR. We also studied the antibiotic susceptibility patterns of the isolates to check for the emergence of any resistant strain.

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