Home > Journals > Journal of Pharmaceutical and Medicinal Chemistry

Journal of Pharmaceutical and Medicinal Chemistry

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Stability Indicating RP-HPLC Method for Simultaneous Estimation of Metformin and Ertugliflozin

A Lakshmana Rao , A Lakshmana Rao1 , U Krishnaveni2

Author Information

Licence:



Journal of Pharmaceutical and Medicinal Chemistry 5(2):p 65-73, July-December 2019. | DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.5219.1

How Cite This Article:

A Lakshmana Rao, U Krishnaveni. Stability Indicating RP-HPLC Method for Simultaneous Estimation of Metformin and Ertugliflozin. J Pharmaceut Med Chem. 2019;5(2):65-73.

Timeline

Received : N/A         Accepted : N/A          Published : N/A

Abstract

A simple, rapid, precise and accurate stability indicating reverse phase high performance liquid chromatography (HPLC) method was developed for the simultaneous estimation of Metformin and Ertugliflozin. Isocratic separation was achieved on Denali C18 (150 x 4.6 mm, 5 μm) column with mobile phase comprising of 0.01 N KH2PO4: acetonitrile (60:40 V/V), pH adjusted 5.4 with 0.01% ortho phosphoric acid. The flow rate was maintained at 1 mL/min and analytes were screened with UV detector at 224 nm. The method was validated according to ICH guidelines with respect to linearity, accuracy, precision and specificity. The drugs were exposed to various stress conditions like, acid, alkali, oxidation, thermal, UV and neutral and the stressed samples were analysed by the proposed method. No co-eluting, interfering peaks from excipients, impurities were observed during stress conditions and all the degraded peaks are well resolved from parent peaks.

Keywords: Metformin; Ertugliflozin; Validation; HPLC.

References

No records found.


About this article

Cite this article

A Lakshmana Rao, U Krishnaveni. Stability Indicating RP-HPLC Method for Simultaneous Estimation of Metformin and Ertugliflozin. J Pharmaceut Med Chem. 2019;5(2):65-73.

Licence:



Received Accepted Published
N/A N/A N/A
DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.5219.1

Keywords


Article Level Metrics

Last Updated

Monday 22 June 2026, 06:16:25 (IST)

437

Accesses

2
28
00

Citations


NA
NA
NA

Download citation


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article

Reprint Order Form
Buy this Article
Institutional Subscriptions

Share