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RP – HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan, Amlodipine and Hydrochlorothiazide in Bulk and Tablet Dosage Forms

Gandla Kumara Swamy, Associate Professor & H.O.D, Department of Pharmaceutical Analysis, Care College of Pharmacy, Oglapur (Vill), Damera(Mdl), WarangalRural (Dist), Telangana 506001, India. , Gandla Kumaraswamy* , B. Sowmya* , K. Pranay*

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Journal of Pharmaceutical and Medicinal Chemistry 3(2):p 89-97, Jul-Dec 2017. | DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.3217.4

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Abstract

A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Irbesartan, Amlodipine besylate and hydrochlorothiazide in bulk and Pharmaceutical formulation using RPC18 Column. The mobile phase (Acetonitrile: methanol: 50mM phosphate buffer adjusted to pH 3.2 with orthophosphoric acid) was pumped at a flow rate of 1.2 mL/min in the ratio of 20:50:30% v/v and the eluent was monitored at 250 nm. Linearity was obtained in the concentration range of 5 30 µg/ml for Amlodipine Besylate, 20 120 µg/ml for Irbesartan and 10 60 µg/ml for Hydrochlorothiazide. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining Irbesartan, Amlodipine and hydrochlorothiazide in bulk and in pharmaceutical dosage form.

Keywords: RPHPLC Method, Irbesartan, Amlodipine besylate, and Hydrochlorothiazide Development and Validation. 

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DOI: DOI: http://dx.doi.org/10.21088/jpmc.2395.6615.3217.4

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