P.K. Shukla Dean, Deparment of Poultry Science, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura 281001, Uttar Pradesh, India., India
Srishtipriya Prasad PhD Scholar, Department of Animal Nutrition, India
Amitav Bhattacharyya Associate Professor, Department of Poultry Science, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura 281001, Uttar Pradesh, India., India
Address for correspondence: P.K. Shukla, Dean, Deparment of Poultry Science, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura 281001, Uttar Pradesh, India., India E-mail: pksmathura@yahoo.co.in
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
Prasad S, Shukla PK, Bhattacharyya A. Recent technologies of in-ovo feeding in poultry. Indian J Agric Bus. 2023;9(2):71–78.
Timeline
Received : June 05, 2023
Accepted : July 31, 2023
Published : August 30, 2023
Abstract
In ovo feeding is a novel technique used to deliver various biologics and nutraceuticals directly
to poultry embryos prior to hatching. The purpose of in-ovo feeding is to improve the overall
health and performance of the hatched chicks. These injections are timed based on the embryonic
development stage and are usually performed on day 18 or 19 of the incubation period. Early
nutritional programming (in ovo injection) is one of the newest and most successful embryos
feeding methods to prepare chickens with healthy intestines, good microbiota, enhanced
immunity and improved overall growth performance. Nutraceuticals including amino acids,
vitamins and carbohydrates were typically employed for early feeding via the in ovo method,
along with feed additives like hormones, medications, exogenous enzymes, herbal plants and
their derivatives, immunostimulants, vaccines and other biological substances. The main focus
of current reviewis to provide an insight into applications and available findings related to in
ovo feeding on performance and health parameters of poultry, along with challenges and future
perspectives of this technique.
References
1. Aladi N, Omede A, Princewill O, Ifeanyi Charles O. In ovo nutrition: Application in poultry reproduction. Res J Poult Sci. 2018;11(1):1-7.
2. Al-Zghoul MB, Dalab AE, Ababneh MM, Jawasreh KI, Al Busadah KA, Ismail ZB. Thermal manipulation during chicken embryogenesis results in enhanced hsp70 gene expression and the acquisition of thermotolerance. Res Vet Sci. 2013;95(2):502-507.
3. Araújo ICS, Café MB, Mesquita MA, Caiado BN, Faria AM, Mello HHC, et al. Effect of a commercial product containing canthaxanthin for in ovo feeding to broiler embryos on hatchability, chick quality, oxidation status, and performance. Poult Sci. 2020;99(11):5598-5606.
4. Baykalir Y, Mutlu SI, Erisir Z. The effects of in-ovo injected d-glucose monohydrate and ascorbic acid on hatchability, body weight and early post-hatch performance of geese. Eur J Vet Med. 2021;1(1):1-6.
5. Berrocoso J, Kida R, Singh A, Kim Y, Jha R. Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken. Poult Sci. 2017;96(6):1573-1580.
6. Bhanja SK, Rath PK, Goel A, Mehra M, Dhara SK, Paswan VK, et al. In ovo nano-silver and nutrient supplementation improves immunity and resistance against Newcastle disease virus challenge in broiler chickens. Front Vet Sci. 2022;9:948069.
7. De Oliveira JE, van der Hoeven-Hangoor E, van de Linde IB, Montijn RC, van der Vossen JM. In ovo inoculation of chicken embryos with probiotic bacteria and its effect on posthatch Salmonella susceptibility. Poult Sci. 2014;93(4):818-829.
8. Fatemi SA, Alqhtani AH, Elliott KEC, Bello A, Levy AW, et al. Improvement in the performance and inflammatory reaction of Ross 708 broilers in response to the in ovo injection of 25-hydroxy vitamin D3. Poult Sci. 2021;100(1):138-146.
9. Filho A, Ferket P, Malheiros R, Bruno de Oliveira CJ, Aristimunha P, Wilsmann D, et al. Enrichment of the amnion with threonine in chicken embryos affects the small intestine development, ileal gene expression and performance of broilers between 1 and 21 days of age. Poult Sci. 2018;98(3):1414-1424.
10. Gao T, Zhao MM, Li YJ, Zhang L, Li JL, Yu LL, et al. Effects of in ovo feeding of L-arginine on the development of digestive organs, intestinal function and post-hatch performance of broiler embryos and hatchlings. J Anim Physiol Anim Nutr (Berl). 2018;102(1):e166-e175.
11. Hu Y, Feng Y, Ding Z, Lv L, Sui Y, Sun Q, et al. Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes. Poult Sci. 2020;99(6):3111-3120.
12. Kermanshahi H, Ghofrani Tabari D, Khodambashi Emami N, Daneshmand A, Ibrahim S. Effect of in ovo injection of threonine on immunoglobulin A gene expression in the intestine of Japanese quail at hatch. J Anim Physiol Anim Nutr (Berl). 2017;101(1):10-14.
13. Kop-Bozbay C, Ocak N. In ovo injection of branched-chain amino acids: Embryonic development, hatchability and hatching quality of turkey poults. J Anim Physiol Anim Nutr (Berl). 2019;103(4):1135-1142.
14. Kucharska-Gaca J, Kowalska E, Jawor M. In ovo feeding – Technology of the future – A review. Ann Anim Sci. 2017;17(4):979-992.
15. Li S, Zhi L, Liu Y, Shen J, Liu L, Yao J, et al. Effect of in ovo feeding of folic acid on the folate metabolism, immune function and epigenetic modification of immune effector molecules of broiler. Br J Nutr. 2016;115(3):411-421.
16. Lu P, Morawong T, Molee A, Molee W. Influences of L-arginine in ovo feeding on the hatchability, growth performance, antioxidant capacity, and meat quality of slow-growing chickens. Animals (Basel). 2022;12(3):392.
17. Mahjar N, Al-Salhie K. The effects of in ovo injection of garlic (Allium sativum L.) extract on hatchability, liver enzymes and antioxidant status of broiler chickens. Basrah J Agric Sci. 2022;35(1):61-70.
18. Arain MA, Nabi F, Marghazani IB, Hassan FU, Soomro H, Kalhoro H, et al. In ovo delivery of nutraceuticals improves health status and production performance of poultry birds: a review. Worlds Poult Sci J. 2022;78(3):765-788.
19. Oliveira G, McManus C, Salgado C, dos Santos V. Bibliographical mapping of research into the relationship between in ovo injection practice and hatchability in poultry. Vet Sci. 2023;10(4):296.
20. Pandey KK, Koley S, Ojha BK, Kurechiya N, Singh S, Singh A. In ovo feeding: View points on the current status, application and prospect in poultry. Indian J Anim Health. 2021;60(2):172-182.
21. Reicher N, Melkman-Zehavi T, Dayan J, Wong EA, Uni Z. Nutritional stimulation by in-ovo feeding modulates cellular proliferation and differentiation in the small intestinal epithelium of chicks. Anim Nutr. 2022;8:91-101.
22. Roto SM, Kwon YM, Ricke SC. Applications of in ovo technique for the optimal development of the gastrointestinal tract and the potential influence on the establishment of its microbiome in poultry. Front Vet Sci. 2016;3:63.
23. Siwek M, Slawinska A, Stadnicka K, Bogucka K, Dunislawska A, et al. Prebiotics and synbiotics – in ovo delivery for improved lifespan condition in chicken. BMC Vet Res. 2018;14(1):402.
24. Slawinska A, Zampiga M, Sirri F, Meluzzi A, Bertocchi M, Tavaniello S, et al. Impact of galactooligosaccharides delivered in ovo on mitigating negative effects of heat stress on performance and welfare of broilers. Poult Sci. 2020;99(1):407-415.
25. Subramaniyan SA, Kang DR, Park JR, Siddiqui SH, Ravichandiran P, Yoo DJ, et al. Effect of in ovo injection of L-arginine in different chicken embryonic development stages on post-hatchability, immune response, and Myo-D and myogenin proteins. Animals (Basel). 2019;9(6):357.
26. Tasharofi S, Mohammadi F, Amiri N, Nazem M. Effects of intra-yolk-sac injection of dextrose and albumin on performance, jejunum morphology, liver and pectoral muscle glycogen and some serum metabolites of broilers. J Anim Physiol Anim Nutr (Berl). 2018;102(4):917-923.
27. Xu QQ, Zhang XY, Zou XT, Dong XY. Effects of in ovo injection of L-histidine on hatch performance and post-hatch development in domestic pigeons (Columba livia). Poult Sci. 2019;98(8):3194-3203.
28. Zhai W, Rowe DE, Peebles ED. Effects of commercial in ovo injection of carbohydrates on broiler embryogenesis. Poult Sci. 2011;90(6):1295-1301.
Data Sharing Statement
There are no additional data available. All raw data and code are available upon request.
Funding
This research received no funding.
Author Contributions
Whether all authors contributed significantly to the work and approve its publication.
Ethics Declaration
This article does not involve any human or animal subjects, and therefore does not require ethics approval.
Acknowledgements
We would like to express our gratitude to the patients, their families, and all those who have contributed to this study.
Conflicts of Interest
The authors report no conflicts of interest in this work.
About this article
Cite this article
Prasad S, Shukla PK, Bhattacharyya A. Recent technologies of in-ovo feeding in poultry. Indian J Agric Bus. 2023;9(2):71–78.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.