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Isolation and Identification of Non-Fermenting Gram Negative Bacteria

Sheetal Sharma, Antim Rewapati, Abhishek Chourasia, Kirti null

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Journal of Microbiology and Related Research 10(2):p 63-67, July-December 2024. | DOI: https://doi.org/10.21088/jmrr.2395.6623.10224.2

How Cite This Article:

Antim Rewapati, Abhishek Chourasia, Kirti, Sheetal Sharma, Isolation and Identification of Non-Fermenting Gram Negative Bacteria. J Microbiol Relat Res. 2024;10(2):63–67.

Timeline

Received : August 26, 2024         Accepted : November 11, 2024          Published : December 25, 2024

Abstract

Background: In medical microbiology, Non-Fermentative group of bacteria have been incriminated as emerging opportunistic pathogens, especially from hospital settings. Non fermenting gram negative bacilli are a group of heterogeneous, aerobic (grow in presence of oxygen), non sporing bacteria. Materials & Methods: 150 isolates from various age groups of both male and female patients were included in the study. A detailed history was elicited and the clinical specimens were collected under aseptic precautions and subjected to preliminary biochemical test and further speciation was done. Results: In the present study Pseudomonas aeruginosa, Acinetobacter baumanii, Pseudomonas fluorescens were isolated of which Pseudomonas aeruginosa Was the most commonest. The non fermenting gram negative bacilli were Isolated 28. 68% from local wound infection, 22. 3% from post operative wound infection, 19. 9% from respiratory tract infections, 8. 37% from gastrointestinal tract infections, 6. 22% from urinary tract infections and 4. 68% from septicemia cases. Conclusion: The non fermenting gram negative bacilli infection is mainly seen in patients with serious underlying risk factors like prolonged stay in hospital, catheterization, underlying diseases like diabetes, malignancies and chronic pulmonary disease. Ciprofloxacin, Ofloxacin, Amikacin, Imipenem appeared to be effective drugs in treating non fermenting gram negative bacilli infections.


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Data Sharing Statement

There are no additional data available. All raw data and code are available upon request.

Funding

This research received no funding.

Author Contributions

All authors contributed significantly to the work and approve its publication.

Ethics Declaration

This article does not involve any human or animal subjects, and therefore does not require ethics approval.

Acknowledgements

We would like to express our gratitude to the patients, their families, and all those who have contributed to this study.

Conflicts of Interest

No conflicts of interest in this work.


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Cite this article

Antim Rewapati, Abhishek Chourasia, Kirti, Sheetal Sharma, Isolation and Identification of Non-Fermenting Gram Negative Bacteria. J Microbiol Relat Res. 2024;10(2):63–67.


Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.


Received Accepted Published
August 26, 2024 November 11, 2024 December 25, 2024

DOI: https://doi.org/10.21088/jmrr.2395.6623.10224.2

Keywords

Non Fermenting Gram Negative BacilliAntimicrobial Susceptibility

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Received August 26, 2024
Accepted November 11, 2024
Published December 25, 2024

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Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.


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