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In-vitro Antioxidant Activity of Hydroalcoholic Extract of Leaves of Colocasia Esculenta Linn

Kalariya Manisha V. , Kalariya Manisha V. , Prajapati Rakesh P. , Sheth Navin R.

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Journal of Pharmaceutical and Medicinal Chemistry 1(1):p 5-11, January - June 2015. | DOI:

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Abstract

Context: Although Colocasia esculenta Linn. (Araceae) commonly known as patarveliya in Guajarati possesses several medicinal properties, a little is known about its antioxidant activity. Objective: The current research was designed to examine the antioxidative potential of hydroalcoholic extract of leaves of Colocasia esculenta (HECE) for the first time using several in-vitro analytical methods. Materials and methods: The antioxidant activity of HECE was evaluated using reducing power ability, nitric oxide scavenging assay and the estimation of total phenolic contents. Results and Discussion: The HECE gave an IC50 value of 45.75±0.38 µg/ml. The reducing power was investigated by Fe3+-Fe2+ transformation in the presence of extract tested using ascorbic acid as standard. The HECE showed increase in reducing ability with increase in concentration at 700nm. The total antioxidant capacity by nitric oxide scavenging method is expressed as curcumin equivalents. The content of total phenolics in HECE was found to be 49.21 ìg gallic acid equivalent/mg. Conclusions: Based on the above results, the higher the phenolic content, the higher the antioxidant capacity was very well  

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