Abstract

Blood systemic infections (BSIs) are major threat in hemodialysis patients. BSIs are diagnosed by blood culturing for bacteria and serology for virus infections. These two methods are time taking and expensive and not covering all BSI microbes. An attempt is made to compare microbial culturing and viral screening with serology by ELISA with blood metagenomic sequencing and sequence analysis. In blood agar media culturing 7 microbes (6 bacterial and 1 fungus), in ELISA screening 0 viruses and in metagenomic sequence analysis 24 microbes (19 bacteria and 1 fungus, 3 viruses, 1 mycoplasma) were detected. Unculturable bacteria are also detected by metagenomic sequence analysis. Hence metagenomic sequence analysis can be best as method for effective detection of BSI’s.

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