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Indian Journal of Genetics and Molecular Research 14(1):p 7-11, Jan-June 2025. | DOI: 10.21088/ijgmr.2319.4782.14125.1
Review Article
An Overview of CRISPR-Cas9 Technology
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Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
Indian Journal of Genetics and Molecular Research 14(1):p 7-11, Jan-June 2025. | DOI: 10.21088/ijgmr.2319.4782.14125.1
How Cite This Article:
Bharati K. An overview of CRISPR-Cas9 technology. Ind J Genet Mol Res. 2025;14(1):7-11.Timeline
Received : March 08, 2025
Accepted : May 03, 2025
Published : June 14, 2025
Abstract
CRISPR-Cas9 is a Nobel Prize winning groundbreaking technology that allows precise editing of an organism’s genome, including that of humans. This technology can specifically modify target DNA sequences, thereby improving its action. The Cas9 enzyme and guide RNA are vital components of the CRISPR-Cas9 complex. The two main mechanisms responsible for its action are non-homologous end joining (NHEJ) and homology-directed recombination (HDR). There are several strategies that can be employed to deliver the CRISPR components into target cells. These include viral vectors, lipofection, electroporation, microinjection, and using nanoparticles. This robust technology has important applications in medicine and agriculture.
References
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Data Sharing Statement
There are no additional data available.
Funding
No funding was received for preparing the manuscript.
Author Contributions
All authors contributed significantly to the work and approve its publication.
Ethics Declaration
This article does not involve any human or animal subjects, and therefore does not require ethics approval.
Acknowledgements
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Conflicts of Interest
There is no conflict of interest.
About this article
Cite this article
Bharati K. An overview of CRISPR-Cas9 technology. Ind J Genet Mol Res. 2025;14(1):7-11.
Licence:
Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
| Received | Accepted | Published |
|---|---|---|
| March 08, 2025 | May 03, 2025 | June 14, 2025 |
DOI: 10.21088/ijgmr.2319.4782.14125.1
Keywords
CRISPR-Cas9Gene editingGenomeGuide RNAMicroinjectionViral vectorLipofectionNanoparticleElectroporationSearch for Similar Articles
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Timeline
| Received | March 08, 2025 |
| Accepted | May 03, 2025 |
| Published | June 14, 2025 |
licence
Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
