Indian Journal of Pathology: Research and Practice

Introduction: The application of C3d and C4d as a diagnostic adjunct in the cases of inflammatory skin disease has been rarely reported in the literature. The purpose of our study is to define the significance of C3d and C4d deposition by an immunohistochemistry (IHC) on paraffin-embedded, formalin-fixed tissue as a correlative diagnostic adjunct in the assessment of inflammatory skin disease. Objective: To evaluate use of C3d and C4d by immunohistochemistry (IHC) on formalin-fixed tissue as diagnostic adjunct in the various inflammatory skin diseases. Method: 1) Diagnosed cases of inflammatory skin diseases on histology were selected for the study. 2) H&E slides were studied for morphological assessment. 3) IHC makers for C3d and C4d were performed on the paraffin blocks using C3d and C4d- antibody based detection kits. Result: 100% cases of DLE & SLE were positive for deposition of C3d & C4d at dermo-epidermal junction. 100% cases of Bullous Pemphigoid & 57% of cases of Bullous Pemphigoid were positive for C3d and C4d deposition respectively at dermo-epidermal junction. 100% cases of Behçet's disease & 42% cases ofLeukocytoclastic Vasculitis were positive for deposition of C3d & C4d in blood vessel. Conclusion: The C3d and C4d assays define an important diagnostic adjunct in the evaluation of collagen vascular disease, cutaneous vascular disease, and autoimmune vesiculobullous disease. The assays may prompt further DIF testing or, in some instances, may even define a reasonable substitute for DIF and/or add to the morphologic assessment of a biopsy specimen submitted for routine light microscopic assessment.

The incidence of solitary thyroid lesions are more common now a days. There is a chance of increase in malignancy rate especially in solitary nodules. Always there will be diagnostic dilemma in differentiating benign and malignant lesions. Our study mainly focused to find the expression of galectin 3 in differential diagnosis of solitary thyroid lesions in a teritiary care hospital for a period of 2 years.

Wilson disease is an uncommon inherited disorder of copper metabolism leading to extensive damage to vital organs like liver and brain. Severe hemolytic anemia is an unusual complication of Wilson’s disease. We present a case of Coomb’s negative acute hemolytic anemia as the presenting manifestation of Wilson’s disease which makes this case worth reporting.

Introduction: Mucopolysacchridosis is a lysosomal storage disorder due to deficiency of lysosomal hydrolase class of enzymes mucopolysaccharidases. It is rare, inherited autosomal recessive disorder results from abnormal accumulation of undegraded GAGs in lysosome of various tissues. Patient Info: Skeletal abnormalities, macroglossia, facial dysmorphia, Scoliosis, excessive salivation are few of the characteristic symptoms which make one to suspect this disorder. We present 9 year old male child having macroglossia and hyper salivation. Diagnosis: He was diagnosed as a case of Mucopolysacchridosis after Berry’s Toluidine blue spot test and urine electrophoresis results.