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Journal of Biochemistry and Biophysics 10(1):p 7-9, Jan - June 2025. | DOI: https://doi.org/10.21088/rfpjbb.2582-3558.10125.1
Review Article
The Biochemistry of DNA Fingerprinting: A Comprehensive Review
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Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
Journal of Biochemistry and Biophysics 10(1):p 7-9, Jan - June 2025. | DOI: https://doi.org/10.21088/rfpjbb.2582-3558.10125.1
How Cite This Article:
Alpana Saha. The Biochemistry of DNA Fingerprinting: A Comprehensive Review. RFP Jour. of Bio. and Biophy. 2025; 10(1): 07–09.Timeline
Received : February 24, 2025
Accepted : June 06, 2025
Published : June 30, 2025
Abstract
DNA fingerprinting, a revolutionary technique in molecular biology, has transformed the fields of forensic science, paternity testing, and genetic research since its inception. This review explores the principles, methodologies, and applications of DNA fingerprinting, highlighting its significance in identifying individuals based on unique genetic patterns. The technique primarily relies on analyzing variable number tandem repeats (VNTRs) and short tandem repeats (STRs), which exhibit high polymorphism among individuals. Advances in polymerase chain reaction (PCR) and capillary electrophoresis have enhanced the accuracy, sensitivity, and efficiency of DNA profiling. In forensics, DNA fingerprinting has become indispensable for solving crimes, exonerating the innocent, and identifying victims of mass disasters. In medicine, it aids in diagnosing genetic disorders and establishing familial relationships. Despite its widespread utility, challenges such as ethical concerns, data privacy, and technical limitations persist. Emerging technologies, including next-generation sequencing (NGS) and CRISPR-based systems, promise to further refine DNA fingerprinting, expanding its applications in personalized medicine and biodiversity conservation. This review underscores the transformative impact of DNA fingerprinting while addressing its limitations and future prospects.
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Data Sharing Statement
There are no additional data available. All raw data and code are available upon request.
Funding
This research received no funding.
Author Contributions
All authors contributed significantly to the work and approve its publication.
Ethics Declaration
This article does not involve any human or animal subjects, and therefore does not require ethics approval.
Acknowledgements
We would like to express our gratitude to the patients, their families, and all those who have contributed to this study.
Conflicts of Interest
No conflicts of interest in this work.
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Cite this article
Alpana Saha. The Biochemistry of DNA Fingerprinting: A Comprehensive Review. RFP Jour. of Bio. and Biophy. 2025; 10(1): 07–09.
Licence:
Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
| Received | Accepted | Published |
|---|---|---|
| February 24, 2025 | June 06, 2025 | June 30, 2025 |
DOI: https://doi.org/10.21088/rfpjbb.2582-3558.10125.1
Keywords
DNA fingerprintingVNTRsSTRsForensic sciencePaternity testingPCRGenetic polymorphismCapillary electrophoresisNext-generation sequencingEthical concernsArticle Level Metrics
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Timeline
| Received | February 24, 2025 |
| Accepted | June 06, 2025 |
| Published | June 30, 2025 |
licence
Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
