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Pulp Volume: An Indepth Tool in Age Estimation a Comparative Retrospective Forensic based Cone Beam CT

Sindhuja. N null, Harsha Vardhan B G, Saraswathi K Gopal

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Indian Journal of Forensic Odontology 16(1):p 19-27, January-June 2023. | DOI: https://doi.org/10.21088/ijfo.0974.505X.16123.2

How Cite This Article:

Sindhuja N, Harsha Vardhan BG, Gopal SK. Pulp volume: An indepth tool in age estimation a comparative retrospective forensic based cone beam CT. Indian J Forensic Odontol. 2023;16(1):19–27.

Timeline

Received : May 31, 2023         Accepted : June 27, 2023          Published : June 30, 2023

Abstract

Background: The field of forensics utilizes various craniofacial structures and skull in identifying an unknown deceased victim. This identification deals with assessing the gender and age of skeletonized remains based on eliciting the ethnicity of the population at archaeological sites, and comparison of post-mortem records with the presumed antemortem records. Interestingly, even a single tooth can be used to assess an age of an individual and this is widely used in forensics for investing legal matters as well as in scientific research purpose. Teeth are resistant to environmental insults and post-mortem decomposition and hence can be retained without distortion.

Aim and Objectives: To analyse the volumetric data of canine and first molars in cone beam CT for estimating age among samples of various age groups and to compare between those values to evaluate which tooth indexed volumetric data gives more specificity in age estimation.

Methodology: It is a retrospective Institutional based Forensic study conducted using 180 samples from 90 full skull CBCT images whose age ranged between 20-65 years, acquired from the dental archives of department of Oral Medicine and Radiology which are equally divided among both genders. Further the samples were categorized into three groups as (20 -35), (36-50) and (51- 65) years in both the gender. All data samples were assessed using the ITK –SNAP 3.8.0 software. Using semi-automatic active contour segmentation method, the volumes of pulps of upper canine and upper first molar were analysed and calculated data were statistically analysed using discriminant functional analysis and multivariate regression analysis to evaluate the correlation of pulp volumes with respect to chronological age.

Results: The multivariate regression analysis done for estimating age among the given groups of samples using upper canine gave statistically highly significant p value of 0.001,0.004 and 0.039 for group I (20-35 years), group II (36-50 years) and group III (51-65 years) respectively. The regression analysis done using upper first molar for age estimation gave statistically highly significant p value of 0.001 for group I, whereas group II and group III gave out insignificant results. The comparative analysis between multivariate Regression analysis for age estimation of upper canine and upper first molar to determine which tooth index has more specificity, gave statistically significant results for canine samples in all age groups. The samples were also analysed for Pearson correlation in estimating age, which resulted in high correlation in group I samples for both canine and first molar, moderate correlation for group II and group III samples for canine pulp volumes and low correlation for group II and group III for molar pulp volumes.

Conclusion: Over the years, CBCT has evolved a lot and today they are being used in various sectors of dentistry. In the field of forensics, various oral and maxillofacial structures are being used as an adjuvant in personnel identification with more precision using cone beam CT. The results of the present study show statistically highly significant correlation between age and upper canine samples in all the age groups analysed. Hence, based on the results of present study, the upper canine can be used as a promising adjuvant tool in forensic odontology for age estimation.


References

  • 1.   Shah P, Velani PR, Lakade L, Dukle S. Teeth in forensics: A review. Indian J Dent Res. 2019;30(2):291-9.
  • 2.   Divakar KP. Forensic odontology: The new dimension in dental analysis. Int J Biomed Sci. 2017 Mar;13(1):1-5.
  • 3.   Erbudak HÖ, Ozbek M, Uysal S, Karabulut E. Application of Kvaal et al.’s age estimation method to panoramic radiographs from Turkish individuals. Forensic Sci Int. 2012;219(1-3):141-6.
  • 4.   Lewis JM, Senn DR. Dental age estimation utilizing third molar development: a review of principles, methods, and population studies used in the United States. Forensic Sci Int. 2010 Sep 10;201(1-3):79-83.
  • 5.   Venkatesh E, Snehal VE. Cone beam computed tomography: basics and applications in dentistry. J Istanbul Univ Fac Dent. 2017;51(3 Suppl 1):S102-S121.
  • 6.   Divakar KP. Forensic odontology: The new dimension in dental analysis. Int J Biomed Sci. 2017;13(1):1-5.
  • 7.   Nagammai N, Saraswathi Gopal K, Srividhya S. Tooth coronal pulp index as a tool for age estimation: An institutional based retrospective cone beam computed tomography study. Indian J Forensic Odontol. 2019;12(2):35-43.
  • 8.   Kvaal SI, Kolltveit KM, Thomsen IO, Solheim T. Age estimation of adults from dental radiographs. Forensic Sci Int. 1995;74(3):175-85.
  • 9.   Cameriere R, Ferrante L, Cingolani M. Variations in pulp/tooth area ratio as an indicator of age: a preliminary study. J Forensic Sci. 2004;49(2):317-9.
  • 10.   Shah PH, Venkatesh R. Pulp/tooth ratio of mandibular first and second molars on panoramic radiographs: An aid for forensic age estimation. J Forensic Dent Sci. 2016;8(2):112.
  • 11.   Biuki N, Razi T, Faramarzi M. Relationship between pulp-tooth volume ratios and chronological age in different anterior teeth on CBCT. J Clin Exp Dent. 2017 May 1;9(5):e688-e693.
  • 12.   Izham A, Auerkari EI. The use of radiology CBCT in odontology forensic. In: AIP Conference Proceedings. AIP Publishing LLC; 2021. p. 030018.
  • 13.   De Vos W, Casselman J, Swennen GR. Cone-beam computerized tomography (CBCT) imaging of the oral and maxillofacial region: a systematic review of the literature. Int J Oral Maxillofac Surg. 2009;38(6):609-25.
  • 14.   Ge ZP, Ma RH, Li G, Zhang JZ, Ma XC. Age estimation based on pulp chamber volume of first molars from cone-beam computed tomography images. Forensic Sci Int. 2015;253:133.e1-7.
  • 15.   Kumar S, Setty J. Age estimation using pulp chamber volume of first molars from cone beam computed tomography images in Indian population. Int J Sci Res. 2016;5(6):2421-5.
  • 16.   Yousefi F, Lari S, Shokri A, Hashemi S, Hosseini M. Age estimation based on the pulp chamber volume of multi-rooted teeth using cone beam computed tomography. Avicenna J Dent Res. 2020;12(1):19-24.

Data Sharing Statement

There are no additional data available. All raw data and code are available upon request.

Funding

This research received no funding.

Author Contributions

Whether all authors contributed significantly to the work and approve its publication.

Ethics Declaration

This article does not involve any human or animal subjects, and therefore does not require ethics approval.

Acknowledgements

We would like to express our gratitude to the patients, their families, and all those who have contributed to this study.

Conflicts of Interest

No conflicts of interest in this work.


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Cite this article

Sindhuja N, Harsha Vardhan BG, Gopal SK. Pulp volume: An indepth tool in age estimation a comparative retrospective forensic based cone beam CT. Indian J Forensic Odontol. 2023;16(1):19–27.


Licence:

Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.



Received Accepted Published
May 31, 2023 June 27, 2023 June 30, 2023

DOI: https://doi.org/10.21088/ijfo.0974.505X.16123.2

Keywords

Pulp VolumeCBCTOral and Maxillofacial RadiologyForensic OdontologyAge

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Received May 31, 2023
Accepted June 27, 2023
Published June 30, 2023

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Attribution-Non-commercial 4.0 International (CC BY-NC 4.0)

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.



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