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Evaluation of Antioxidant Activity of Amalakayas Rasayana in Tissue Homogenate of Albino Rats

Samarakoon S.M.S , Samarakoon S.M.S , Chandola H.M , Ravishankar B , Herapathdeniya S.K.M.K , Varun B. Gupta

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Indian Journal of Ancient Medicine and Yoga 5(1):p 19-25, January - March 2012. | DOI:

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Abstract

 This study was designed to evaluate the antioxidant activity of classical poly-herbal compound Ayurvedic formulation “Amalakayas Rasayana” (AR) on experimental animals. AR was administered in standard dose orally for seven consecutive days prior to forced swimming- induced hypothermia and stress ulcers. The antioxidant potential was assessed by determining and comparing the changes in adrenal ascorbic acid, catalase, glutathione, total protein and lipid peroxidation in test drug group and vehicle control group with that of stress control group. Pretreatment with AR and vehicle caused highly significant attenuation of lipid per oxidation (p<0.001) in comparison to stress control group. Both AR and vehicle increased total glutathione content in stomach tissue homogenate in highly significant manner (p<0.001). Moreover, AR attenuated adrenal ascorbic acid (10.47%), catalase (08.11%), and total protein (06.11%), but the values are statistically insignificant. The results suggest that AR possesses significant anti-oxidant activity which could be due to its cytoprotective activity.

Keywords: Amalakayas Rasayana; Antioxidant; Ghee; Honey; Lipid peroxidation.

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