Journal of Pharmaceutical and Medicinal Chemistry

Vaccines are mankind's most powerful weapons against any kind of communicable disease caused by viruses, bacteria, etc. The vaccines are involved in the boosting of the immune system against particular microorganisms. The major problems for the vaccines are temperature maintenance and transportation. The rise in temperature can deteriorate the vaccine and cause it to lose its activity. During the transport of such vaccines, which are highly degradable due to slight temperature changes, extreme caution is exercised. Vaccine stabilization methods are designed to minimize potency loss during manufacture, storage, distribution, and clinical usage. This problem can be solved by using cryogenics, which is an emerging technology used in various fields to store food preparations, biological cell cultures, transportation of vaccines, aerospace sectors, and agriculture areas. In cryogenics, the temperatures are kept very low by using cryogens like oxygen, nitrogen, helium and hydrogen. In general, vaccines like COVID are going to be stored at the possible lowest temperatures like 2-8°C, where as the Ebola vaccine is –60°C to –80°C. The employment of cryogenics can even achieve the temperature –273°C i.e., the absolute zero

A wide variety of chemicals may cause cutaneous and ocular burns and systemic effects either by absorption or inhalation, requiring in most of the cases medical and/or surgical treatment. Given the nature of the agents involved and the type of injuries (depth, lung injury, eye involvement, etc.) they produce a relevant loss of working time. Long term sequelaeare, unfortunately, not uncommon.

A simple, accurate, precise RP-HPLC method was developed for the simultaneous estimation of the Metformin and Empagliflozin in the tablet dosage form. Materials and Methods: Chromatogram was run through Std Agilent 18 (150 x 4.6mm, 5μm). Mobile phase taken as 0.1% OPA Buffer: Acetonitrile in 60:40% v/v ratio, and pumped through the column at a flow rate of 1mL/min. The buffer used in this method was 0.1% OPA buffer. The temperature was maintained at 25°C. Results and Discussion: Optimized wave length selected was 245 nm. The retention times of Metformin and Empagliflozin were found to be 2.193 min and 2.668 min respectively. %RSD of the Metformin and Empagliflozin was found to be 1.4 and 0.8 respectively. % Recovery was obtained as 99.66% and 100.24% for Metformin and Empagliflozin respectively. LOD, LOQ values obtained from regression equations of Metformin and Empagliflozin were 0.02, 1.48, and 0.05, 4.93 respectively. Regression equation of Metformin is y = 20952x + 9914.5 and y = 41842x + 571.79 of Empagliflozin. Retention times were decreased and that run time was decreased, The Reverse Phase HPLC isocratic method for Metformin and Empagliflozin is developed and validated as per ICH guidelines. Conclusion: The test method is found to be sensitive, accurate, precise, linear, convenient, and economical that can be adopted in regular quality control tests in Industries..