Journal of Microbiology and Related Research

 Not Provided.

 Fungal prosthetic joint infections are rare; with Candida albicans being the most frequently reported pathogen in English literature. Immunocompromised state, as well as extensive use of the newer triazole, fluconazole to suppress fungal infections has lead to an increase in fungaemia due to albicans and non-albicans candida. Here, we report a case of disseminated Candida krusei, in an immunocompromised patient with total hip replacement one month prior following fracture neck of left femur. Patient was deemed infection free following prosthetic joint removal, thorough debridement and antifungal therapy.

Key words: Candida krusei, Prosthetic Joint Infections, fungaemia , Biofilm

Background: Shewanella putrefaciens is as yet rarely responsible for clinical syndromes in humans. It is a gram negative non fermentative, oxidase positive, motile bacillus that is found mainly in marine environments. It produces hydrogen sulfide. It is a facultative anaerobe which grows quickly on both solid and liquid media. It was first isolated from dairy products in 1931 by Derby and Hammer. Aim : Infections with Shewanella putrefaciens in humans is on the rise in recent years and it mainly causes soft tissue infections, otitis media, bacteremia and pneumonia with other pathogens like E.coli. Materials and Methods: Here we report a case of septicaemia caused by Shewanella putrefaciens in a 9 month old infant. . Blood sample was collected in paediatric blood culture broth in a ratio of 1:5 (blood: broth) and was incubated in BactT/Alert (Biomeriux) and then subcultured on blood and MacConkey agar plates. The growth of gram negative,non fermentative,oxidase positive bacteria was identified as Shewanella putrefaciens by Microscan (Siemens) and was found to be susceptible to Amoxy/clav,cefipime, c e f o p e r a z o n e / s u l b a c t u m , c e f o t a x i m e , c e f t a z i d i m e , ciprofloxacillin.doripenum,ertapenem,imipenemmeropenem,piperacillin/tazobactum,tetracycline,ticarcillin/clavulinic acid,tigecycline and Trimethoprim/sulpha. Conclusion: Shewanella putrefaciens is as yet rarely responsible for clinical syndrome in humans. However the infection with this organism is on the rise in recent years. So attention should be devoted to unusual pathogens.

Keywords: Shewanella putrefaciens antibiotic susceptibility, identification, infections, septicaemia.

Present short work was designed to study incidences of Salmonella species in the raw seafood from local markets and fish collection jetties of Goa. Study observed 12% (24/200) of the raw sea foods (9.5% fish, 2% prawns and 0.5% crabs) contaminated with Salmonella spp. All the 24 isolates were tested for antimicrobial susceptibility by disc diffusion assay. Isolates were found resistant to trimethoprim-sulfamethoxazole (100%), ampicillin (41%) and tetracycline (25%). All the isolates were sensitive to ciprofloxacin, chloramphenicol, streptomycin and norfloxacin. Intermediate phenotypes were observed against nalidixic acid, kanamycin and gentamicin.The study shows occurrence of antibiotic resistant Salmonella species in retail fish markets and collection jetties in Goa, India.  

Keywords: Bluetongue virus serotptype-1, cytopathic effect, Phylogenetic analysis, vp2 gene 

 Nitric oxide (NO) is formed in gastrointestinal tract by both intestinal mucosa and commensal microbiota. In this work, NO production by probiotic bacteria Lactobacillus plantarum 8PA3 was demonstrated by electron paramagnetic resonance (EPR)and NOspecificfluorescent dyes DAF-FM DA (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) and DAA (1,2-diaminoanthraquinone sulfate). After we ruled out the generation of NO via denitrification, we showed that NO production by L. plantarumdepends on L-arginine what is typical for NO-synthases (NOS), and thus proposed NOS-activity in L. plantarum. However,we were unable to inhibit the NO production with three specific NOS inhibitorsL-NAME, L-NIL, and nNOS Inhibitor I.We also demonstrated an increased level of NO production in lactobacilli with damaged cellular membranes suggesting stress-dependent NO production by L. plantarum.