Abstract

DNA analysis is used in forensic investigations to identify individuals and solve crimes. Important pieces of evidence are frequently found at crime scenes, including bloodstains. However, both the quantity and the quality of the extracted DNA can vary depending on the conditions in which bloodstains are exposed before analysis, such as different temperatures. This study, to evaluate the genome potential for performing short tandem repeat (STR) analysis on bloodstains collected from three different temperature settings: -200, 40, and room temperature. The study will evaluate how temperature affects DNA extraction and typing. Previous studies have demonstrated that burns and high heat can cause DNA to degrade and lower DNA quantities in bloodstains. Additionally, research has shown that even after being heated to specific temperatures, cleaned bloodstains can still produce sufficient DNA for analysis. This research will help to clarify the challenges and limitations involved in conducting STR analysis on bloodstains exposed to a range of temperatures by examining the effects of various temperatures on DNA quantity and quality in bloodstains. The results will help investigators in the forensic field increase DNA recovery techniques and raise the accuracy of DNA profiling in criminal investigations.

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Data Sharing Statement

Funding