Abstract

The advancement of forensic DNA analysis has revolutionized criminal investigations, providing crucial evidence for identifying suspects and establishing their involvement in various crimes. The extraction of DNA from blood stains is a fundamental step in forensic investigations, enabling the profiling of individuals for identification and genetic analysis. The objective of this research is to evaluate and compare the DNA extraction methods on different fabric types, which is cotton, and silk. Blood stains were spotted in both the cloths and incubated for a certain period of time. After which, DNA extraction was done using phenol chloroform method. The quality and quantity of the extracted DNA are assessed using established techniques such as Gel electrophoresis and qPCR amplification. The results of the experiments indicate variations in the efficiency of DNA extraction methods depending on the fabric type. Ideally both the fabrics have shown similar pattern of DNA recovery but Cotton fabrics demonstrate higher DNA recovery rates, of DNA recovery but Cotton fabrics demonstrate higher DNA recovery rates, compared to silk fabric but this is non-significant. In conclusion, provides valuable insights into the challenges and opportunities associated with DNA extraction from blood stains on different fabrics for forensic investigation profiling. The research findings contribute to the advancement of forensic DNA analysis and offer practical guidance for forensic scientists to optimize their methods when handling blood-stained fabric evidence. Further research and development in this field will undoubtedly strengthen the forensic community’s ability to solve crimes and administer justice with greater accuracy and reliability.

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