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Speciation and Resistotyping of Coagulase Negative Staphylococci in Clinical Isolates in Kerala Medical College, Palakkad

Assistant Professor Department of Medical Microbiology, Kerala Medical College, Palkkad, Kerala 679503, India. , Rudra Murthy K.G.* , Gundala Obulesu** , Padmavathi-*** , Arun Arvnd****

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Journal of Microbiology and Related Research 3(2):p 89-96, Jul-Dec 2017. | DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3217.7

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Abstract

Introduction: Coagulase negative staphylococci are important causative agents of 10% of pyogenic infections in hospitals, which include a range of infections like surgical wound sepsis, bacteremia, native valve endocarditis (NVE) and prosthetic valve endocarditis, osteomylitis, pyoarthritis, peritonitis, mediastinitis, prostatitis, infection of vascular grafts and pacemakers. Materials and Methods: 105 samples are collected from infected post operative cases, infected burns, infected wounds from traumotology unit, diabetic foot, gangrene, nonhealing ulcers, pyoderma, and impetigo, patients with intravenous cannulae. Results: of 105 samples tested 50 (47.61%) of CNS are isolated, Out of 50 isolates of Coagulase negative staphylococci 24 (48%) isolates were identified as S. epidermidis. Out of 34 community isolates, 13 (38.23%) were sensitive to Penicillin and. 11(61.76%) were resistant 13 (75.47%) were sensitive to Oxacillin and 3(13.23%) were resistant. Conclusion: In the present study CNS was the most predominant organism (105/50; 47.61%). Most of CNS isolates were from the hospital infections (68%). Coagulase negative Staphylococci have been increasingly gaining importance in hospital infections, compared to E. coli, Klebsiella, MRSA and Pseudomonas. Majority of the isolates were from IV catheters (76.19%), stressing the need for more aseptic precautions in ICU settings.

Keywords: I.V.; MRSA; CNS. 

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DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3217.7

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