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Separation and Detection of Quizalofop-Ethyl Herbicide by Thin-Layer Chromatography

Mali Bhagwat D , Mali Bhagwat D

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Red Flower's Journal of Forensic Chemistry and Toxicology 5(2):p 111-113, July-Dec 2019. | DOI: http://dx.doi.org/10.21088/jfct.2454.9363.5219.3

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Mali Bhagwat D. Separation and Detection of Quizalofop-Ethyl Herbicide by Thin-Layer Chromatography. J Forensic Chemistry Toxicol. 2019;5(2):111-113.

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Abstract

Herbicides are frequently used in agriculture for control of weeds in crops. Quizalofop-ethyl is a postemergence herbicide widely used for control of grassy weeds in crops, intended for human consumption. The general population may be exposed to quizalofop-ethyl via ingestion of food or drinking water. Sometimes it is misused for suicidal poisoning. A systematic analysis of herbicides acting as poison in human body is carried out by forensic toxicologists. Their method involves screening of poison followed by its instrumental assay. Therefore a simple, rapid, sensitive and reliable thin- layer chromatographic method for detection of quizalofop-ethyl is presented. The quizalofop-ethyl contain quinoxaline ring in its structure having –N=C-CH=N --group. These react with Dragendorff`s reagent to give orange-red coloured spot in yellow background. The detection limit was found to be 10 μg per spot (20 μg/cm2). The constituents of viscera (amino acids, peptides, proteins, etc.) do not interfere in the test.

 

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Mali Bhagwat D. Separation and Detection of Quizalofop-Ethyl Herbicide by Thin-Layer Chromatography. J Forensic Chemistry Toxicol. 2019;5(2):111-113.

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DOI: http://dx.doi.org/10.21088/jfct.2454.9363.5219.3

Keywords

Herbicides; Quizalofop-ethyl; Thin-Layer Chromatography; Spray Reagent; Dragendorff`s.

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