Nand Lal Dean, Department of Life Science and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 208024, Uttar Pradesh, India., India
Address for correspondence: Nand Lal, Dean, Department of Life Science and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 208024, Uttar Pradesh, India., India E-mail: nl_pr@yahoo.co.in
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Lal N. Phytotoxic effects of cobalt, copper and cadmium on seed germination and seedling vigour in mungbean [Vigna radiata (L) Wilczek]. Indian J Biol. 2023;10(2):59-76.
Timeline
Received : June 05, 2023
Accepted : July 31, 2023
Published : August 30, 2023
Abstract
Investigations were carried to study the influence of 0 (control), 0.5, 1.0, 2.0, 4.0, 6.0 and 8.0 mM of cadmium (Cd++), cobalt (Co++), and copper (Cu++) ions in an aqueous solution (as chloride salts) on germination, root elongation, hypocotyl growth, mobilization efficiency, tolerance index and vigour index of mungbean (Vigna radiata (L.) Wilczek) variety K-851 seeds and seedlings. The extent of inhibition of germination was found to depend on, metal concentration, metal type and incubation period. In comparison to control, there was a complete inhibition of root and hypocotyl growth in germinating mung bean seeds at Co and Cu concentration ≥ 6.0 mM. The order of these metals on inhibition of germination, root elongation and hypocotyl growth was Cu > Cd > Co. The phytotoxic effects of tested metal ions were also evident in case of mobilization efficiency, tolerance index and vigour index. All the tested metal concentration could be efficiently tolerated by V. radiata seedlings. Variation in these physiological parameters has role in combating the metal chlorides mediated stress, and could be used for measuring the phytotoxicity and degree of tolerance of the test system.
References
1. Abdul-Baki AA, Anderson JD. Vigour determination in soybean seeds by multiple criteria. Crop Sci 1973;13: 630-633.
2. Arduini I, Godbold DL, Onnis A. Cadmium and Copper change root growth and morphology of Pinus pinea and Pinus pinaster seedlings. Physiol Plant 1994; 92: 675-680.
3. Bazzaz FA, Rolfe GL, Windle P. Differing sensitivity of corn and soybean photosynthesis and transpiration to lead contamination. J Environ Qual 1974; 3: 156-158.
4. Hernandez LE, Carpenaruiz R, Garate A. Alterations in the mineral nutrition of Pea seedlings exposed to Cadmium. J Plant Nutr 1996; 19: 1581-1598.
5. Hernandez LE, Garate A, Carpenaruiz R. Effects of cadmium on the uptake, distribution and assimilation of nitrate in Pisum sativum. Plant Soil 1997; 189: 97-106.
6. Lal N. Molecular Mechanisms and Genetic Basis of Heavy Metal Toxicity and Tolerance in Plants. In: Plant Adapatation and Phytoremediation, Ashraf M, Ozturk M, Ahmad MSA (Eds)., Springer-Verlag+ Buisiness Media B.V., Dordrecht, (2010) pp 35-58.
7. Lal N, Mishra R. Phytotoxic effects of lead on biomass and photosynthetic pigments in gram (Cicer arietinum L.) seedlings. J EcoPhysiol 2004; 7: 51-55.
8. Lal N, Mishra R. Effect of Cadmium on germination, growth and photosynthetic pigments in Cowpea. Indian J Pulses Res 2006; 19: 76-78.
9. Liu J, Reid RJ, Smith FA. The mechanism of Cobalt toxicity in mungbeans. Physiol Plant 2000; 110: 104-110.
10. Mohan R, Singh R, Singh PR, Saran B. Effects of GA3 against Cd toxicity during germination in black gram. Proc 4th APPSC Confi g. and Neo Botanica Conv., Patna, India (eds.) Mehta AS and Saran B (1996) pp 87-90.
11. Malone C, Koeppe DE, Miller RJ. Localization of lead accumulated by corn plants. Plant Physiol 1974; 53: 388-394.
12. Mishra A, Choudhuri MA. Amelioration of lead and mercury effects on germination and rice seedling growth by antioxidants. Biol Plant 1998; 41: 469-473.
13. Munzuroglu O, Geckil H. Effects of metals on seed germination, root elongation, and Coleoptile and hypocotyls growth in Triticum aestivum and Cucumis sativus. Arch Environ Contam Toxicol 2002; 43: 203-213.
14. Nedelkoska TV, Doran PM. (2000) Characteristics of metal uptake by plants species with potential for phytoremediation and phytomining. Minerals Eng 2000; 13: 549- 561.
15. Oncel I, Keles Y, Ustun AS. Interactive effects of temperature and metal stress on the growth and some biochemical compounds in wheat seedlings. Environ Pollut 2000; 107: 315-320.
16. Van Assche F, Clijsters H. Effects of metals on enzyme activity in plants. Plant Cell Environ 1990; 13: 195-206.
17. Wierzbicka M, Obidzinska J. The effect of lead on seed inhibition and germination in different plant species. Plant Sci 1998; 137: 155-171.
Data Sharing Statement
There are no additional data available. All raw data and code are available upon request.
Funding
This research received no funding.
Author Contributions
Whether all authors contributed significantly to the work and approve its publication.
Ethics Declaration
This article does not involve any human or animal subjects, and therefore does not require ethics approval.
Acknowledgements
We would like to express our gratitude to the patients, their families, and all those who have contributed to this study.
Conflicts of Interest
The authors report no conflicts of interest in this work.
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Cite this article
Lal N. Phytotoxic effects of cobalt, copper and cadmium on seed germination and seedling vigour in mungbean [Vigna radiata (L) Wilczek]. Indian J Biol. 2023;10(2):59-76.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
Percent germination of V. radiata cv. K-851 in the presence of varying concentrations of Cobalt, Copper and Cadmium.
Description: No description available.
Growth response of root, shoot and hypocotyl of germinating seeds of V. radiata cv. K-851 in the presence of varying concentrations of Cobalt, Copper and Cadmium
Description: No description available.
Variation in Mobilization efficiency, Tolerance index and Vigour index of seedlings of V. radiata cv. K-851 in the presence of varying concentrations of Cobalt, Copper and Cadmium.