Abstract

The present investigation was conducted in the year 2020-2021 at the Department of Plant Protection, ASPEE College Horticulture, Navsari Agricultural University, Navsari with an aim to evaluate the impact of highly pathogenic isolates on seed germination, root length, shoot length and seedling vigour index of bottle gourd seeds var. ABG-1. Total ten seed mycoflora were isolated using agar plate method, standard blotter method and deep-freezing method viz., Aspergillus niger, Aspergillus flavus, Alternaria alternata, Colletotrichum sp., Curvularialunata, Fusarium oxysporum, Nigrospora oryzae, Penicillium sp., Rhizoctonia sp., and Rhizopus stolonifera. After the pathogenicity teston seeds of bottle gourd var. ABG-1 under in vitro (standard paper towel) and in vivo (pot method) condition, Alternaria alternata, Colletotrichum sp. Curvularia lunata, Fusarium oxysporum and Rhizopus stolonifer were evaluated to check their impact on the seed germination, shoot length, root length and seedling vigour using paper towel method. Result revealed that the seed inoculate with Fusarium oxysporum caused significantly lower per cent of germination (22.00%), shoot and root length (1.74cm, 4.31cm) and seedling vigour index 133.67 followed by Colletotrichum sp. (25.00%, 1.89cm, 4.10cm and 149.60).

• 1.   Abdelwehab, S. A.; El-Nagerabi, S. A. F. and Elshafie, A. E. (2014). Mycobiota associated with imported seeds of vegetable crops in Sudan. Open Mycol. J.,8: 156-173.
• 2.   Ahmed, M. I. and Reddy, R. (1993). A pictorial Guide to the identification of seed borne fungi of sorghum, pearl millet, finger millet, chickpea, pigeonpea and groundnut. Information Bulletin No., 34 pp: 132.
• 3.   Anonymous (2019). Area and Production. Retrieved from https://doh.gujarat.gov.in /Accessed 24 Nov, 2020.
• 4.   Anonymous (2024). Largest Bottle Gourd Producing State in India. Retrieved from https://currentaffairs.adda247.com/largestbottle-gourd-producing-state-in-india/?utm_ source=chatgpt.com Accessed on 16 July, 2025.
• 5.   Avinash, T. S. and Rai, V. R. (2013). Identification of diverse fungi related with selected cucurbitaceae vegetables. J. Agril. Technol., 9 (7):1837-1848.
• 6.   Gajera, R. R. and Joshi, D. C. (2016). Effect of seed borne fungi on germinating seeds in their biocontrol in maize. J. Environ. Sci. and Natural resour., 5 (1): 117-120.
• 7.   Jogi, M. G.; Padule D. N. And Kamdi, S. R. (2010). Detection of seed mycoflora of chilli and it’s impact on seed germination and seedling vigour. Inter. J. Pl. Sci., 5 (2): 502-504.
• 8.   Neergaard P. (1977). “Seed Pathology” Vol. I and II. The MacMillan Press Ltd., London and Basigstoke., pp. 1187.
• 9.   Rahman, A. H. M. M.; Anisuzzaman, M.; Ahmed, F.; Islam, A. K. M. R. and Naderuzzaman, A. T. M. (2008). Study of nutritive value and medicinal uses of cultivated Cucurbits. J. Appl. Sci. Res., 4 (5): 555-558
• 10.   Shah, N. and Jiskani, M. M. (2014). Pathogenicity test and inoculum level of Fusarium wilt of bottle gourd (Lagenaria siceraria). Persian Gulf Crop Prot., 3 (3): 54-62.
• 11.   Sharma, D. K.; Sharma, N. and Pareek, A. (2014). Seed borne, post-harvest diseases of round gourd (Citrullus vulgaris var. Fistulosus) and its nutritive value. Asian J. Plant Pathol., 8 (2): 30-33.
• 12.   Soni, N. (2017). Investigation on fruit and seed mycoflora of bottle gourd and its management. Thesis M.Sc. (Horti.). Chaudhary Charan Singh Haryana Agricultural University, Hisar (Haryana). 25-49 p.
• 13.   Sultana, N. and Ghaffar, A. (2009). Seed borne fungi associated with bottle gourd (Lagenaria Siceraria (Mol.) Standl. Pak. J. Bot., 41 (1): 435-442.
• 14.   Suprava, S. (2017). Seed mycoflora, pathogenicity and control of pathogens of spinach. Thesis M.Sc. (Botany), Tribhuvan University Kirtipur, Kathmandu, Nepal. 66 p.
• 15.   Thakur, R. P.; Gunjotikar, G. A. and Rao, V. P. (2010). Safe movement of ICRISAT’s seed crops germplasm, published by ICRISAT, Hyderabad, pp. 80-194.

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