Abstract

Introduction: The peptides resulting from the collagen degradation by collagenases have wide range of applications including wound healing. However, most of the wound healing studies involve collagenase obtained from anaerobic and pathogenic microorganisms thus increasing the production cost of the same. Methodology: Earlier in our laboratory, collagenase was isolated and purified from aerobic, non-pathogenic Bacillus altitudinis and it has shown excellent activity in vitro. Thus, the present study was carried out to evaluate the wound healing potential of collagenase isolated and purified from Bacillus altitudinis in murine model. For this, male mice were divided in to 2 groups viz. test group (n=6) and control group (n=6) and burn wound was created on the dorsal side of each mouse with the help of a heated brass bar after exposing the skin. In case of test group purified collagenase (10µg/100 µl) was applied on the burn wound while phosphate buffered saline (100 µl) was applied in case of control group for 5 consecutive days. On day 6th the mice (n =3) were sacrificed from each group and wound biopsies were collected for histopathological examinations Results: Histopathological examinations showed the absence of epithelium in control group whereas in test group initial signs of regeneration of epidermis were observed. The wound recovery when examined visually and by calculating wound area by using calliper the result showed that the wound was fully recovered. Conclusion: Purified collagenase can be used as therapeutic measure in burn wound healing.

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