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Detection and Molecular Characterization of Indian Isolates of Canine Parvovirus in Fecal Samples

Prasad Minakshi* , Prasad Minakshi* , Koushlesh Ranjan** , Supriya* , Basanti Brar* , Gaya Prasad***

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Journal of Microbiology and Related Research 2(2):p 83-88, July - December 2016. | DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.2216.3

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Abstract

 The canine parvovirus which causes myocarditis and enteritis is considered to be a very serious pathogen causing mortality in pups. The original strain CPV2 is found to be continuously evolving and presently three antigenic strains CPV­2a, CPV­2b and CPV­2c found to be circulating worldwide. In the present study 52 fecal samples were collected from 1­2 month old pups showing clinical signs of CPV from Hisar (India) and screened using vp2 gene specific seminested polymerase chain reaction assay. Three fecal samples were found positive for CPV infection. The PCR products were allowed for nucleic acid sequencing and nucleotide sequences were deposited to GenBank with accession numbers KC012923, KC012928 and KC012932. The sequence analysis using various bioinformatics tools revealed that all the three isolates belong to CPV­2a genotype and are much closer to other CPV­2a genotype from India and China. The present study showed that the predominant genotype running in India is CPV2a. Therefore, CPV2a strain should be incorporated in vaccine formulation.

Keywords: CPV; CPV­2a; PCR; Genotype.

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DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.2216.3

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