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Indian Journal of Agriculture Business 1(1):p 29-36, Jan-Jun 2015. | DOI:
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Detection and Diagnosis of Strain Variation in Canine Parvoviral Infections in Dogs
Deepikakumari G. , Deepikakumari G. , J. Dhanalakshmi , Y. N. Reddy
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Indian Journal of Agriculture Business 1(1):p 29-36, Jan-Jun 2015. | DOI:
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Abstract
A total of 10 clinical samples and one known control sample in the form of rectal swabs werecollected from dogs showing signs of diarrhoea. The samples were clarified, processed and subjected to haemagglutination, rapid immunochromatography kit test and polymerase chain reaction assays. Of the 10 samples screened all (100%) were positive for CPV infections. Isolation of virus from the clarified faecal samples was done in both CRFK and MDCKcell lines. With one of the positive faecal sample SDS –Page was carried out. Strain variation was detected of which 10 samples belonged to CPV 2a and one sample belonged to CPV 2b by PCR assay with CPV-2ab primers and 2b primers. Partial sequencing based on VP2 gene was carried out with one of the CPV 2a isolate and phylogenetic tree was constructed to know the homology with other Indian isolates.
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