Home > Journals > Journal of Microbiology and Related Research

Journal of Microbiology and Related Research

Submit your article Read this Journals View all journals
Harish

Editorial Board Member

Dr. Harish Shukla

  • Address : Assistant Professor, Biochemistry, North-eastern Hill University, India
  • E-Mail : harishshukla@nehu.ac.in

Research Interests :


Introduction


I am Dr. Harish Shukla, an Assistant Professor in the Department of Biochemistry at North-Eastern Hill University (NEHU), Shillong, India. My academic and research interests lie at the interface of structural biology, biophysics, bioinformatics, and molecular microbiology, with a particular focus on understanding the structure–function relationships of proteins involved in infectious diseases. My research integrates computational approaches, including molecular docking, molecular dynamics simulations, protein structure prediction, and bioinformatics, with experimental biophysical techniques to investigate biomolecular interactions and identify potential therapeutic targets. I am especially interested in intrinsically disordered proteins, protein folding, molecular recognition, recombinant protein expression and purification, and the structural characterization of proteins from pathogenic microorganisms such as Mycobacterium tuberculosis. Alongside research, I am actively involved in teaching postgraduate courses in biochemistry, biophysics, research methodology, and biostatistics, with an emphasis on developing analytical and critical-thinking skills among students. I have supervised postgraduate dissertations and doctoral research scholars and remain committed to fostering a collaborative and interdisciplinary research environment. I also contribute to the scientific community through editorial and peer-review activities for international journals and by organizing national and international conferences and academic workshops. My broader goal is to bridge computational and experimental biology to advance our understanding of biomolecular systems and facilitate the development of novel diagnostic and therapeutic strategies. Through research, teaching, and academic leadership, I strive to contribute meaningfully to scientific innovation, capacity building, and the advancement of higher education while promoting collaborative research at both national and international levels.


AFFILIATION


Assistant Professor

Biochemistry

North-eastern Hill University

Umshing Mawkynroh

Meghalaya

India

793022

Email : harishshukla@nehu.ac.in


RESEARCH IDS



RESEARCH INTERESTS


# Area
1. Microbiology
2. Molecular Biology
3. Parasitology
4. Structural Biology

PAPERS PUBLISHED


# Article Title Year
1. Subedi, S.; Shukla, H.; Uversky, V. N.; Tripathi, T. Physical Principles and Molecular Interactions Underlying Protein Phase Separation. The Three Functional States of Proteins, 2025, 197–212 2025
2. Nag, N.; Shukla, H.; Uversky, V. N.; Tripathi, T. Targeting Phase-Separated Protein States for Drug Discovery. The Three Functional States of Proteins, 2025, 255–273. 2025
3. Nag, N., Shukla, H., Padhi, A. K., & Tripathi, T. (2023). Comprehensive analysis of liquid-liquid phase separation propensities of HSV‐1 proteins and their interaction with host factors. Journal of Cellular Biochemistry. 2023
4. Shukla, R., Shukla, H., & Tripathi, T. (2021). Structure-based discovery of phenyl-diketo acids derivatives as Mycobacterium tuberculosis malate synthase inhibitors. Journal of Biomolecular Structure and Dynamics, 39(8), 2945-2958. 2021
5. Tripathi, T., Ghosh, A., Todur, V. N., Kalita, P., Rajendran, V., Kalita, J., Shukla, R., Chetri, P. B., Shukla, H., Sonkar, A., Lyngdoh, D. L., Singh, R., Chikara, S. K., & Tripathi, T. (2020). Draft genome of the liver fluke Fasciola gigantica. ACS Omega, 5(19), 11084-11091. 2020
6. Yadav, S., Prakash, J., Shukla, H., Das, K. C., Tripathi, T., & Dubey, V. K. (2020). Design of a multi-epitope subunit vaccine for immune protection against the Leishmania parasite. Pathogens and Global Health, 114(8), 471-481. 2020
7. Kalita, P., Das, K. C., Shukla, H., & Tripathi, T. (2019). Conserved Arg451 residue is critical for maintaining the stability and activity of thioredoxin glutathione reductase. Archives of Biochemistry and Biophysics. https://doi.org/10.1016/j.abb.2019.108098 2019
8. Gupta, S., Shukla, H., Kumar, A., Shukla, R., Kumari, R., Tripathi, T., Singh, R. K., & Anupurba, S. (2019). Mycobacterium tuberculosis nucleoside diphosphate kinase shows interaction with putative ATP binding cassette (ABC) transporter, Rv1273c. Journal of Biomolecular Structure and Dynamics, 1-11. https://doi.org/10.1080/07391102.2019.1595150 2019
9. Lyngdoh, D. L., Shukla, H., Sonkar, A., Anupam, R., & Tripathi, T. (2019). Portrait of the Intrinsically Disordered Side of the HTLV-1 Proteome. ACS Omega, 4(6), 10003-10018. 2019
10. Kalita, P., Lyngdoh, D. L., Padhi, A. K., Shukla, H., & Tripathi, T. (2019). Development of multi-epitope driven subunit vaccine against Fasciola gigantica using immunoinformatics approach. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2019.07.024 2019
11. Sonkar, A., Shukla, H., Shukla, R., Kalita, J., & Tripathi, T. (2019). Unfolding of Acinetobacter baumannii MurA proceeds through a metastable intermediate: A combined spectroscopic and structure computational investigation. International Journal of Biological Macromolecules, 126, 941-951. 2019
12. Vijayakumar, R., Shukla, R., Shukla, H., & Tripathi, T. (2018). Structure-function studies of the asparaginyl tRNA synthetase from Fasciola gigantica: Understanding the role of catalytic and non-catalytic domains. Biochemical Journal, 475(21), 3377-3391. 2018
13. Sonkar, A., Lyngdoh, D., Shukla, R., Shukla, H., Tripathi, T., & Ahmed, S. (2018). Point mutation A394E in the central intrinsic disordered region of Rna14 leads to chromosomal instability in fission yeast. International Journal of Biological Macromolecules, 119, 785-791. 2018
14. Kalita, P., Shukla, H., Shukla, R., & Tripathi, T. (2018). Biochemical and thermodynamic comparison of the selenocysteine containing and non-containing thioredoxin glutathione reductase of Fasciola gigantica. Biochimica et Biophysica Acta (BBA) - General Subjects, 1862(6), 1306-1316. 2018
15. Rajendran, V., Kalita, P., Shukla, H., Kumar, A., & Tripathi, T. (2018). Aminoacyl-tRNA synthetases: Structure, function, and drug discovery. International Journal of Biological Macromolecules, 111, 400-414. 2018
16. Tripathi, T., Ghosh, A., Todur, V. N., Kalita, P., Rajendran, V., Kalita, J., Shukla, R., Chetri, P. B., Shukla, H., Sonkar, A., Lyngdoh, D. L., Singh, R., Chikara, S. K., & Tripathi, T. (2018). Draft genome of the liver fluke Fasciola gigantica. bioRxiv. https://doi.org/10.1101/451476 2018
17. Shukla, H., Khan, S. R., Shukla, R., Krishnan, M. Y., Akhtar, M. S., & Tripathi, T. (2018). Alternate pathway to ascorbate induced inhibition of Mycobacterium tuberculosis. Tuberculosis, 111, 161-169. 2018
18. Shukla, H., Khan, S. R., Shukla, R., Krishnan, M. Y., Akhtar, M. S., & Tripathi, T. (2018). Alternate pathway to ascorbate induced inhibition of Mycobacterium tuberculosis. Tuberculosis, 111, 161-169. 2018
19. 19. Shukla, R., Shukla, H., & Tripathi, T. (2018). Activity loss by H46 mutation in Mycobacterium tuberculosis isocitrate lyase is due to decrease in structural plasticity and collective motions of the active site. Tuberculosis, 108, 143-150. 2018
20. 20. Shukla, R., Shukla, H., Sonkar, A., Pandey, T., & Tripathi, T. (2018). Structure-based screening and molecular dynamics simulations offer novel natural compounds as potential inhibitors of Mycobacterium tuberculosis isocitrate lyase. Journal of Biomolecular Structure and Dynamics, 36(8), 2045-2057. 2018
21. Shukla, R., Shukla, H., Kalita, P., & Tripathi, T. (2018). Structural insights into natural compounds as inhibitors of Fasciola gigantica thioredoxin glutathione reductase. Journal of Cellular Biochemistry, 119, 3067-3080. 2018
22. Shukla, R., Shukla, H., Kalita, P., Sonkar, A., Pandey, T., Singh, D. B., Kumar, A., & Tripathi, T. (2018). Identification of potential inhibitors of Fasciola gigantica thioredoxin1: Computational screening, molecular dynamics simulation and binding free energy studies. Journal of Biomolecular Structure and Dynamics, 36(8), 2147-2162. 2018
23. Shukla, R., Shukla, H., & Tripathi, T. (2018). Structural and energetic understanding of novel natural inhibitors of Mycobacterium tuberculosis malate synthase. Journal of Cellular Biochemistry, 120(2), 2469-2482. 2018
24. Shukla, H., & Tripathi, T. (2018). Studying parasite gene function and interaction through ribozymes and riboswitches design mechanism. In S. Singh (Ed.), Synthetic Biology: Omics tools and their applications (pp. 51-74). Springer Singapore. 2018
25. Kalita, J., Shukla, R., Shukla, H., Gadhave, K., Giri, R., & Tripathi, T. (2017). Comprehensive analysis of the catalytic and structural properties of a mu-class glutathione s-transferase from Fasciola gigantica. Scientific Reports, 7, 17547. 2017
26. Pandey, T., Shukla, R., Shukla, H., Sonkar, A., Tripathi, T., & Singh, A. K. (2017). A combined biochemical and computational studies of the rho-class glutathione s-transferase sll1545 of Synechocystis PCC 6803. International Journal of Biological Macromolecules, 94, 378-385. 2017
27. Singh, A. K., Rastogi, S., Shukla, H., Asalam, M., Rath, S. K., & Akhtar, M. S. (2017). Cdc15 Phosphorylates the C-terminal Domain of RNA Polymerase II for Transcription during Mitosis. Journal of Biological Chemistry, 292(13), 5507-5518. 2017
28. Shukla, H., Shukla, R., Sonkar, A., & Tripathi, T. (2017). Alterations in conformational topology and interaction dynamics caused by L418A mutation leads to activity loss of Mycobacterium tuberculosis isocitrate lyase. Biochemical and Biophysical Research Communications, 490(2), 276-282. 2017
29. Shukla, H., Kumar, R., Sonkar, A., Mitra, K., Akhtar, M. S., & Tripathi, T. (2017). Salt-regulated reversible fibrillation of Mycobacterium tuberculosis isocitrate lyase: Concurrent restoration of structure and activity. International Journal of Biological Macromolecules, 104, 89-96. 2017
30. Shukla, H., Shukla, R., Sonkar, A., Pandey, T., & Tripathi, T. (2017). Distant Phe345 mutation compromises the stability and activity of Mycobacterium tuberculosis isocitrate lyase by modulating its structural flexibility. Scientific Reports, 7, 1058. https://doi.org/10.1038/s41598-017-01235-z 2017
31. Sonkar, A., Shukla, H., Shukla, R., Kalita, J., Pandey, T., & Tripathi, T. (2017). UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) of Acinetobacter baumannii (AbMurA): Structural and functional properties. International Journal of Biological Macromolecules, 97, 106-114. 2017
32. Shukla, H., Kumar, V., Singh, A. K., Rastogi, S., Khan, S. R., Siddiqi, M. I., Krishnan, M. Y., & Akhtar, M. S. (2015). Isocitrate lyase of Mycobacterium tuberculosis is inhibited by quercetin through binding at N-terminus. International Journal of Biological Macromolecules, 78, 137-141. 2015
33. 33. Shukla, H., Kumar, V., Singh, A. K., Singh, N., Kashif, M., Siddiqi, M. I., Krishnan, M. Y., & Akhtar, M. S. (2015). Insight into the structural flexibility and function of Mycobacterium tuberculosis Isocitrate Lyase. Biochimie, 110, 73-80. 2015
34. Shukla, H., Singh, S. K., Singh, A. K., Mitra, K., & Akhtar, M. S. (2015). The C-terminus hot spot region helps in the fibril formation of bacteriophage-associated Hyaluronate Lyase (HylP2). Scientific Reports, 5, 1442. 2015
35. Kalita, P., Shukla, H., Gadhave, K., Giri, R., & Tripathi, T. (2018). Role of the glutaredoxin domain and FAD in the stabilization of thioredoxin glutathione reductase. Archives of Biochemistry and Biophysics, 656, 38-45. 2013
36. Gautam, A., Shukla, H., Khan, S., Rishi, P., & Bhakuni, V. (2010). Structural and functional features of UDP-N-acetylenolpyruvylglucosamine reductase of Mycobacterium tuberculosis H37Rv. International Journal of Integrative Biology. 2010

BOOKS PUBLISHED


# Title Publication Year

BOOK CHAPTERS PUBLISHED


# Chapter Title Book Title Publication Year

ACADEMIC PROFILE


# Examination Passing Year Country
1. Ph.D 2016 India
2. M.Sc 2008 India

EMPLOYMENT DETAILS


# Institution Position Country Service Period
1. North-Eastern Hill University, East Khasi Hills Assistant Professor , Department of Biochemistry India 3 years, 7 months, 15 days
2. North-Eastern Hill University, East Khasi Hills Post-Doctoral Fellow , Department of Biochemistry India 2 years, 11 months, 29 days
3. North-Eastern Hill University, East Khasi Hills Research Associate, Department of Biochemistry India 1 years, 11 months, 29 days
Total Period : 8 years, 7 months, 13 days

PAPERS PRESENTED IN CONFERENCES



RESEARCH PROJECTS



MEMBERSHIPS



TRAINING PROGRAMS ATTENDED



HONOURS AWARDS AND DISTINCTIONS



EVENTS ATTENDED



EVENTS ORGANIZED