RFP Journal of Biochemistry and Biophysics

The Spirometry is the test usually used to diagnose the capacity of lungs. Spirometry which signifies meant the measurement of breath. The Spirometry is routinely performed as part of the pulmonary function tests (PFTs).It is used to measure the Lung Function with specific importance to the amount (volume) and/or speed (flow) of air that can be inhaled and exhaled. The used of this test involved in assessing breathing patterns that identify conditions such as asthma, pulmonary fibrosis, cystic fibrosis, and Chronic obstructive pulmonary disease (COPD). It is also helpful as part of a system of health surveillance, in which breathing patterns are measured over time. The present study focused on the patterns of the Obstructive and Restrictive Impairments among of urban population of Jaipur. The patients selected specifically from the visiting patients with some symptoms to the health camp. In this camp the assessment of Lung Functions was done by using the RMS Helios 401 PC based Spirometer. The parameters, as per the manual are used for the diagnosis of the Obstructive and Restrictive Impairments. The parameter assessed includes Age, Height, Weight, Gender, Smoker/ Non-Smokar, FVC Pred, FVC (M. Pred),% Pred. M.,% Pred, Lung Age (P),Lung Age (M). As per the instrument manual restrictive stage COPD as FEVI/FVC>=70%and FEV 1<80% . In the present study the COPD before the medication mentioned as pre-test is found to be 144 patients in the total numbers of 164 which accounts for 87.80%.

Keywords: Spirometry; Lungs; COPD; FVC; FEVI.

The aim of present study was to study the levels and analyze the variation in glycosylated hemoglobin (HbA1c) levels in Iron Deficiency Anemia (IDA).This study was conducted on 50 cases who were diagnosed to be having iron deficiency anemia and 50 age matched healthy controls which are not having any form of anemia. The levels of HbA1cwas significant increased in cases ofiron deficiency anemia as compared to those in the healthycontrols. There were no differences in the random glucose levels between the anemic and healthy subjects. It is not only the blood sugar levels that affects HbA1c levels but it also may be affected by other factors such as hemoglobinopathies and anemia among which most common in India is iron deficiency anemia. Hence, it very vital to be have an estimate of iron levels in the blood so as to be able to take therapeutic decision to treat diabetes based on HbA1c levels. Therefore, it is always advisable to correct any iron deficiency before any diagnostic or therapeutic decision is made.

Keywords: Glycosylated Hemoglobin; HbA1c; Iron Deficiency Anemia; Diabetes Mellitus.

Present study was aimed to assess lipid profile in diabetic and non-diabetic patients with history of myocardial infarction.

Methods: Study conducted in the Department of Biochemistry, Indian Institute of Medical Science and Research, Warudi, Tq. Badnapur, District Jalna (M.S.)in collaboration with Hedgewar hospital during period of 2015-17. Biochemical investigations include fasting blood sugar, postprandial blood sugar and lipid profile (total cholesterol, triglyceride, LDL-cholesterol, HDL-cholesterol and VLDL).

Results: Present study shows significant increase in the levels of triglyceride, LDL-cholesterol, while cholesterol level increases non-significantly. HDL-cholesterol levels decreased significantly in patients of myocardial infarction (MI) with diabetes mellitus compared to non-diabetic patients with history of myocardial infarction.

Conclusion: Diabetic patients with history of myocardial infarctionwere having significantly deranged lipid parameters and higher risk of dyslipidemic complications as compared to nondiabetic patients with history of myocardial infarction. 

Keywords: AMI (Acute Mycocardial Infarction); HDL (High Density Lipoprotein; LDL (Low Density Lipoprotein); Diabetic & Non-Diabetic.

The extraction and purification of isoflavones from defatted soy flour (DSF) are attempted by leaching and adsorption chromatography. The isoflavones were extracted in methanol and processed employing silica gel column chromatography. After silica gel column chromatography 0.4 mg/ml of isoflavones content (3.9 mg/g DSF) was obtained which has 16.8 mg of total isoflavones. The process helps to the recovery of the isoflavones along with the removal of contaminants up to 90% which confirmed through HPLC.

Keywords: Concentration; Methanol; Isoflavones; Soybean; Column Chromatography.

 

Background: The ability to diagnose and treat subclinical hypothyroidism in diabetic patients may greatly enhance the quality of life. The treatment of hypothyroidism helps in better control of other associated co-morbidities. Therefore, in present study association between thyroid dysfunction and Diabetes was assessed by correlating Fasting plasma glucose and Thyroid profile.

Materials and Methods: A total of 40 subjects attending the OPD who were diagnosed for diabetes were recruited.40 normal age and sex matched participants were recruited as controls. Informed written consent was obtained from all the participants. Fasting plasma glucose was estimated by glucose oxidase method and TSH, T 3 and T4 by enzyme-immunoassay (ELISA) method using commercially available kit. Subjects grouped as normal with TSH level0.39-6.16mIU/l, T3 levels 52-185ng/dl and T4level as 5.0-15.0µg/dl. Lower T 3 and T4 with high TSH is considered as Hypothyroidism and higher levels of T3, T4 with low TSH is considered as hyperthyroidism.The data was analysed using SPSS version 20. Unpaired t test and Pearson’s correlation was performed to find the significant differences between the groups and their correlation. P<0.05 was considered as statistically significant.

Results: The Chi-square test was used to find the association between diabetes and thyroid dysfunction showed significant association (p<0.05) between Diabetes and thyroid dysfunction. On comparison, showed no difference in the sugar levels according to dysfunction status(p<0.05).

Conclusion: A routine assessment of thyroid hormone levels in diabetics is necessary, particularly with subclinical thyroid hormones level.

Keywords: Fasting Plasma Glucose Level; Glucose Oxidase Method; TSH; T3; T4; Enzymelinked Immunoassay.