Description: PCR amplification of the Bt 2Ax strain
Description: Column purification (CP) of PCR amplified products
Description: Concentration checking of isolated Plasmid DNA (pET 28a)
Description: Confirmation of plasmid DNA through Restriction digestion
Description: Concentration checking of vector and insert after
Description: Colony PCR after transformation
Description: Plasmid PCR (pET 28a + 2ax)
Description: Restriction digestion of plasmid DNA (PET 28A+ 2AX)
Description: Protein harvesting through induction from BL 21 transformed with pET 28a + Cry 2Ax
Description: Quantification of 2Ax protein using BSA standard
Description: Bioassay of cry 2Ax protein against pink boll worm (Spodoptera litura)
Description: Bioassay of pink pigmented boll worm (Spodoptera litura ) without any protein (Control)
Description: Bioassay of pink pigmented boll worm (Spodoptera litura ) with Cry 2Ax alone (Heterologus E.coli)
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