Background:Enterococci are important causes of both communities acquired and nosocomial infections. They show intrinsic resistance to a number of commonly used antibiotics, particularly the cephalosporins. During the last few years, Enterococci have acquired resistance to a number of important antibiotics including glycopeptides. Enterococci resistant to all three antimicrobial agents (penicillin, aminoglycosides and Vancomycin) pose a serious challenge not only for clinicians but also for health care institutions. It results in treatment failure, selection and spreading of resistant strains in the health care institution. The increasing occurrence of Enterococcusspecies, worldwide, since late 1980s, is of particular concern due to the emergence of Vancomycin Resistant Enterococci (VRE). VRE has also been reported from some parts of India. The appearance of VRE has limited the therapeutic options available for clinicians. Materials and Methods: Study was carried out in the Department of Microbiology, Shimogga Institute of Medical Sciences, Shimoga, between November 2015 and October 2018. Enterococci were isolated from various clinical samples at a tertiary care hospital using the standard techniques. The isolated Enterococci are then tested for routine antibiotics sensitivity by disc diffusion method including Vancomycin sensitivity. Results and Discussion:A total 330 Enterococcus isolates were obtained from various clinical specimens such as C. Among 330 Enterococcus species, 235 species were Enterococcus faecalis and 95 species are Enterococcus faecium. The Enterococcal species showed 100% sensitivity to Vancomycin and Linezolid. The ability of the laboratory to identify enterococci and to detect Vancomycin resistance promptly and accurately is essential in recognizing VRE colonization and infection and avoiding complex, costly containment efforts that are required when recognition of the problem is delayed. Further, acquisition of Vancomycin resistance leaves few options for therapeutic management. Keywords: Enterococcus Faecalis; Enterococcus Faecium; Vancomycin; Vre.