A non-experimental descriptive study to assess the knowledge and practice about the use of central line associated bloodstream infection care bundle (CLABSI) to prevent central line associated bloodstream infection, among staff nurses working in selected hospitals, Hyderabad, with a view to develop an information booklet with the objectives To assess the knowledge and practice regarding CLABSI bundle,to find out correlation between knowledge and practice among staff nurses, to find out association between knowledge and practice among staff nurses with their selected demographic variables, to develop information booklet on CLABSI bundle for staff nurses. Research design selected for the present study was non experimental descriptive research design. A structured questionnaire was used to collect the data from the staff nurses and an observational CLABSI bundle checklist was used to assess the practice score of the CLABSI bundle practices.Purposive sampling technique was used for the selection of sample. Total sample size for the study was 60. Majority of the staff nurses had below average knowledge on CLABSI bundles that is 45(75%) having below average knowledge, whereas 15 (25%) demonstrated average knowledge. The result of the study is majority of the staff nurses had below average practice to CLABSI bundle that is 48 (80%) scored below average practice scores. 11(18.6%) staff nurses were in average practice scores, whereas 1(1.7%) staff nurse scored above average practice scores. The study shows that there is moderately positive correlation between knowledge and practice among staff nurses, (r=0.4). The study shows that there is no significant association between knowledge and practice regarding CLABSI bundle among staff nurses with selected demographic variables. An information booklet was provided to all intensive care nurses, regarding knowledge and practice of CLABSI bundle and advised to implement CLABSI bundle checklist wherever needed in order to prevent central line associated bloodstream infection.