Bluetongue (BT) is an economically important, vector-borne, non-contagious, viral disease of ruminant animals. It is caused by the Bluetongue virus (BTV) and belongs to the genus Orbivirus under the family Reoviridae. The clinical form of the disease is reported in sheep, white-tailed deer, bighorn sheep, etc. The subclinical infections are reported from camelids, cattle, and goats. Due to its high morbidity, mortality, and huge economic loss to the livestock sector, BT is listed as a multispecies disease by World Organization for Animal Health (OIE). The diagnosis of BTV is required for the identification of etiological agents and control of disease which is essential for the international trade of livestock and its products. Some of the diagnostics techniques such as reverse transcription-polymerase chain reaction (RT-PCR), competitive enzyme-linked immunosorbent assay, agar gel immunodiffusion assay are OIE recommended tests for BTV diagnosis at the international level. For BTV control, serological and vector surveillance, vector control, mass vaccination of susceptible animals, etc. are used.