Microgravity is associated with an increase of peroxidative damage. The effect is more pronounced after long-duration space flight and can even last for several weeks after landing. The extensive research is going on preventation of peroxidative damge due to microgravity. It have been evidence that curcumin (diferuloylmethane), a yellow pigment in curry powder, exhibits antioxidant, anti-inflammatory, and proapoptotic activities. To determine preventive effect of curcumin on peroxidative damage due to two bed rest conditions. 20 healthy male volunteers equally divided into two groups ( 10 with curcumin and 10 without curcumin ) were studied in condition before , during, and just bed rest conditions -6° head-down-tilt (HDT) bed rest and bed rest position (BD) for 10 days. We measured the salivary and serum oxidative markers such as Malonaldehyde, 8-hydroxydeoxyguanosine, vitamin C and E just before HDT & BD, during HDT & BD experiment, and in recovery in with curcumin and without curcumin group. The value of serum and salivary Vitamin C & E showed statistically significant decrease in both bed rest conditions condition as compared to before and in recovery stage , however levels were decreased less in curcumin groups as compared to without curcumin groups ( Table-1&2, P<0.05) .MDA and 8-OHdG levels showed significant increase in simulating microgravity and Zero gravity condition as compared to before and in recovery stage , however levels were low in curcumin grops as compared to without curcumin groups ( Table-1& 2, P<0.05). Serum and salivary correlation analysis revealed strong and highly significant correlation for MDA, Vitamin C & E and 8 dihydro-2 deoxyguanosine (8-OHdG) in before , during and recovery in both bed rest conditions. Since, saliva can be easily collected, non-invasive and measurement of salivary markers levels may prove to useful in space research. Hence, curcumin prevent peroxidative damage in both bed rest condition. Further study is required on antioxidation action of curcumin in space microgravity condition. 

Key Words- Curcumin, Serum, Saliva, oxidative stress, two bed rest position, space microgravity.