Bhadrikadi Ghrita also known as Marmakshata Ghrita is an Ayurvedic herbal formulation specially indicated for pain management in Marmabhighata (Injury to vital structures). Standardization is necessary to ensure the quality, efficacy and uniformity of the products to get a desired action. No research work is carried out in this direction so this article highlights the standardization of Bhadrikadi Ghrita.
Objectives: To standardize Bhadrikadi Ghrita on various parameters, in order to assure its safety and efficacy.
Materials and Methods: The Raw drugs required for the preparation of Ghrita (Herbal Ghee formulation) were procured from KLE GMP certified Ayurveda pharmacy and the Ghrita was prepared as per the Ayurveda classical methods. The Ghrita was analysed for its organoleptic, physico-chemical, Microbial counts, Phyto-constituents and chromatographic analysis to meet the required standards at Central research facility, AYUSH approved central drug testing laboratory of KLE Shri BMK Ayurveda Mahavidyalaya.
Results: The analysed ghrita shown loss on drying (1.143%), Saponification value (292.10), Iodine value (20.827 w/v), Refractive Index at 40 (1.4563), Acid Value (1.560), Specific Gravity (0.9133), pH (6.00).TLC of Ghrita with mobile phase (Toluene: Ethyl Acetate) ratio (7:3) showed refraction value of short wave at 0.17, 0.23, 0.51,0.73 and Long wave at 0.06, 0.11, 0.15, 0.22, 0.28, 0.36, 0.43, 0.47, 0.53, 0.57, 0.73, 0.79,0.85,0.90. The above parameters can be taken as standard for
the analysis of the formulation.
Conclusion: The standardization of any formulation is depended on various parameters like physicochemical, Chromatographic and Microbial count standards which serve as preliminary quality index parameters. The obtained results can be kept as a reference for the assurance of quality control, which would further help in the establishment of quality standards.