Guduchi (Tinospora cordifolia) is described by the synonym of ‘Amrita’, which means it has the capacity of life restoring. So, it is the important Ayurvedic Rasayana drug, or rejuvenative tonics. Guduchi Ghana is rehabilitated form of Kwatha. Guduchi Ghana has mentioned in Sidhdha Yoga Sangraha by the name of  ‘Samshamnivati’ Guduchi Ghana was prepared by Kwatha and Soxhlet method as per above reference and modern technique respectively in the department of Rasashastra and Bhaishajya Kalpana, Jamnagar. The third sample was procured from the market for analytical comparison. All the samples were analyzed physico-chemically i.e. pH, Loss on drying, Acid insoluble ash, Water and Methanol  soluble extractives, qualitative Tests , quantitatively Estimation of alkaloids content and starch, Heavy metal analysis and HPTLC was also carried out. The obtained result of analytical parameters shows  that the water solubility of Kwatha sample, Soxhlet and market sample are 41.16, 90.16 and 93.63 respectively. Glycoside was found to be present in both classical and Soxhlet sample but absent in Market sample. The % of total Alkaloids content in Kwatha, Soxhlet and market sample are 0.080, 0.500 and 0.305 respectively. The % of Starch content in Kwatha sample, Soxhlet and market sample are 0.506, 0.478 and 0.315 respectively

Key words: Guduchi, Kwatha, Ghana, Soxhlet method, Tinosporaside.