Volume
10, Issue 1, January – June 2024, Pages 21-27
Original Article
Blood Metagenomic Sequence Analysis for Evaluation of Chronic Systemic Infections
Mohammed Shakeel1, Ayla Sanjay2, Zainab Kausar3, Vadlamudi Nikhil4, Chand Pasha5
Research Student, Department of Microbiology, Mumtaz degree and PG College Malakpet, Affiliated to Osmania University, Hyderabad, Telangana, 2-4Research Student, Department of Microbiology, Nizam College, Osmania University Hyderabad 500001, Telangana, 5Senior Assistant Professor & Head, Department of Microbiology, Nizam College, Osmania University Hyderabad 500001, Telangana, India
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Blood systemic infections (BSIs) are major threat in hemodialysis patients. BSIs are diagnosed by blood culturing for bacteria and serology for virus infections. These two methods are time taking and expensive and not covering all BSI microbes. An attempt is
made to compare microbial culturing and viral screening with serology by ELISA with blood metagenomic sequencing and sequence analysis. In blood agar media culturing 7 microbes (6 bacterial and 1 fungus), in ELISA screening 0 viruses and in metagenomic
sequence analysis 24 microbes (19 bacteria and 1 fungus, 3 viruses, 1 mycoplasma) were detected. Unculturable bacteria are also detected by metagenomic sequence analysis. Hence metagenomic sequence analysis can be best as method for effective detection of BSI’s.
Keywords : Meta genomic DNA sequence analysis; Systemic infections; Dialysis;
Uncultured Bacteria; Blood infection.
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