Vaccine injections are the most common reason for iatrogenic pain in childhood. With the steadily increasing number of recommended vaccinations, there has been a concomitant increase in concern regarding the adequacy of pain management. In view of this investigator aimed to assess the effect of rhythmic skin tap on pain During DPT vaccination among toddler. It was a quasi-experimental study;  based on Kolcaba Comfort  Theory. After getting informed consent from primary care givers, 60 toddler (30 each in experimental and control group) who attended the immunization OPD of Aswini Hospital was selected as samples. The demographic performa of the toddler was assessed from care giver, immunization card and procedural pain was assessed by standardized FLACC behavioural pain scale. The findings revealed that, the intervention of  rhythmic skin tap was effective in reducing pain with p = <0.001 and there is a significant difference between the post-test pain score among toddlers with p = <0.001. Moreover the analysis states that there is no association between pain among toddlers with selected demographic variable. Study proved that rhythmic skin tap technique during the administration of DPT vaccination resulted in significant reduction in pain level in the experimental group than the control group. Hence it is clear that the rhythmic skin tap technique is an effective measure to reduce pain level during invasive procedure.