Immunization had laid a major development in areas of preventive pediatrics. Immunization had also helped in preventing various childhood diseases and decreasing mortality rates among children. Child Health is a major concern in developing countries like India. The objectives of the study were to assess the attitude regarding of Mother of under five children regarding Immunization, assess the knowledge of Mother of under five children regarding Immunization and to find the association between knowledge and demographical variables. The 60 samples were selected by purposive sampling technique and study was conducted in mau village under mohanalalganj community health center. Three types of tool were used such as Questionnaire to assess demographic variable, Questionnaires for knowledge assessment and a Five point Likert scale for attitude assessment. The results showed that 31.7% had moderate knowledge, 68.3% had inadequate knowledge and none had adequate knowledge. The results also concluded that 70% had moderate attitude and none had good attitude. There was statistical significant association in knowledge level of mothers with age (χ2=5.805*),